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CHIT1 elisa kit :: Human chitinase 1 (chitotriosidase) ELISA Kit

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Catalog # MBS923372
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

chitinase 1 (chitotriosidase) (CHIT1), ELISA Kit

Popular Item
Also Known As

Human chitinase 1 (chitotriosidase) , CHIT1 ELISA Kit

Product Synonym Names
Human chitinase 1 (chitotriosidase) (CHIT1) ELISA kit; CHI3; CHIT; FLJ00314; MGC125322; chitotriosidase; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase 1 (chitotriosidase)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS923372 Testing Data
Sequence Length
447
OMIM
gene 614122
3D Structure
ModBase 3D Structure for Q13231
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CHIT1. No significant cross-reactivity or interference between human CHIT1 and analogues was observed.
Samples
serum, plasma, tissue homogenates, cell culture supernates.
Assay Type
Sandwich (Quantitative)
Detection Range
31.25 pg/ml-2000 pg/ml.
Sensitivity
Typically less than 7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CHIT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCHIT1purchase
MBS923372 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chitinase 1 (chitotriosidase) (CHIT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHIT1. The ELISA analytical biochemical technique of the MBS923372 kit is based on CHIT1 antibody-CHIT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHIT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHIT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Typical Testing Data/Standard Curve (for reference only) of CHIT1 elisa kit
CHIT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS923372. Click to request current manual
NCBI/Uniprot data below describe general gene information for CHIT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,681 Da
NCBI Official Full Name
chitotriosidase-1 isoform 2
NCBI Official Synonym Full Names
chitinase 1 (chitotriosidase)
NCBI Official Symbol
CHIT1  [Similar Products]
NCBI Official Synonym Symbols
CHI3; CHIT; CHITD
  [Similar Products]
NCBI Protein Information
chitotriosidase-1; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase
UniProt Protein Name
Chitotriosidase-1
UniProt Synonym Protein Names
Chitinase-1
Protein Family
UniProt Gene Name
CHIT1  [Similar Products]
UniProt Entry Name
CHIT1_HUMAN
NCBI Summary for CHIT1
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for CHIT1
CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.2.1.14; Hydrolase; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; lysosome

Molecular Function: endochitinase activity; chitin binding; chitinase activity

Biological Process: polysaccharide catabolic process; response to bacterium; immune response; chitin catabolic process

Disease: Chitotriosidase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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