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CHRND elisa kit :: Mouse Acetylcholine ELISA Kit

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Catalog # MBS284149
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CHRND elisa kit
Product Name

Acetylcholine (CHRND), ELISA Kit

Full Product Name

Mouse Acetylcholine receptor subunit delta (CHRND) ELISA Kit

Product Synonym Names
CHR-ND; CHRN-d; NAChRD; N-AChRD; N-AChR-D; ACHRD; CMS2A; FCCMS; SCCMS; Neuronal Acetylcholine Receptor Delta
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P02716
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CHRND. No significant cross-reactivity or interference between Mouse CHRND and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CHRND elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CHRNDpurchase
MBS284149 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholine (CHRND) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHRND. The ELISA analytical biochemical technique of the MBS284149 kit is based on CHRND antibody-CHRND antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHRND antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHRND. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CHRND elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHRND in samples. An antibody specific for CHRND has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRND present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRND is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRND bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for CHRND. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,143 Da
NCBI Official Full Name
acetylcholine receptor subunit delta
NCBI Official Synonym Full Names
cholinergic receptor, nicotinic, delta polypeptide
NCBI Official Symbol
Chrnd  [Similar Products]
NCBI Official Synonym Symbols
Acrd; Achr-4; L10076
  [Similar Products]
NCBI Protein Information
acetylcholine receptor subunit delta
UniProt Protein Name
Acetylcholine receptor subunit delta
Protein Family
UniProt Gene Name
Chrnd  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for CHRND
This gene encodes the delta subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The delta subunit together with the alpha subunit forms the ligand-binding site. [provided by RefSeq, Nov 2012]
UniProt Comments for CHRND
nAChRD: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRND are a cause of multiple pterygium syndrome lethal type (MUPSL). Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent. Defects in CHRND are a cause of congenital myasthenic syndrome slow-channel type (SCCMS). SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes. Defects in CHRND are a cause of congenital myasthenic syndrome fast-channel type (FCCMS). FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Delta/CHRND sub-subfamily.

Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1 C5|1 44.07 cM

Cellular Component: acetylcholine-gated channel complex; cell junction; integral component of membrane; membrane; plasma membrane; postsynaptic membrane; synapse

Molecular Function: acetylcholine binding; acetylcholine-gated cation channel activity; extracellular ligand-gated ion channel activity; ion channel activity; ligand-gated ion channel activity; protein binding

Biological Process: cation transport; ion transport; musculoskeletal movement; neuromuscular synaptic transmission; regulation of membrane potential; response to nicotine; skeletal muscle contraction; skeletal muscle tissue growth; synaptic transmission, cholinergic; transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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