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CHST2 elisa kit :: Mouse carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 ELISA Kit

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Catalog # MBS913065
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Typical Testing Data/Standard Curve (for reference only)
Product Name

carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 (CHST2), ELISA Kit

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Also Known As

Mouse Carbohydrate sulfotransferase 2, CHST2 ELISA Kit

Product Synonym Names
Mouse Carbohydrate sulfotransferase 2 (CHST2) ELISA kit; C6ST; GST2; N-acetylglucosamine 6-O-sulfotransferase; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS913065 Testing Data
Sequence Length
530
3D Structure
ModBase 3D Structure for Q80WV3
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CHST2. No significant cross-reactivity or interference between mouse CHST2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml-1600 pg/ml.
Sensitivity
The minimum detectable dose of mouse CHST2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CHST2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCHST2purchase
MBS913065 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 (CHST2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHST2. The ELISA analytical biochemical technique of the MBS913065 kit is based on CHST2 antibody-CHST2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHST2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHST2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CHST2 elisa kit
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHST2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CHST2 elisa kit
CHST2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS913065. Click to request current manual
NCBI/Uniprot data below describe general gene information for CHST2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,828 Da
NCBI Official Full Name
carbohydrate sulfotransferase 2
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 2
NCBI Official Symbol
Chst2  [Similar Products]
NCBI Official Synonym Symbols
Chts2; GST-2; Gn6st; AI428561; AW121776; GlcNAc6ST; C130041E03Rik
  [Similar Products]
NCBI Protein Information
carbohydrate sulfotransferase 2; gn6st-1; glcNAc6ST-1; N-acetylglucosamine-6-O-sulfotransferase; N-acetylglucosamine 6-O-sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
UniProt Protein Name
Carbohydrate sulfotransferase 2
UniProt Synonym Protein Names
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1; GlcNAc6ST-1; Gn6st-1
UniProt Gene Name
Chst2  [Similar Products]
UniProt Synonym Gene Names
Gst2; GST-2; GlcNAc6ST-1; Gn6st-1  [Similar Products]
UniProt Entry Name
CHST2_MOUSE
UniProt Comments for CHST2
CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Transferase; EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane; trans-Golgi network

Molecular Function: phenanthrol sulfotransferase activity; proteoglycan sulfotransferase activity; trans-3,4-dihydrodiolphenanthrene sulfotransferase activity; 2-phenanthrol sulfotransferase activity; galactose 3-O-sulfotransferase activity; 1-phenanthrol sulfotransferase activity; transferase activity; 3-phenanthrol sulfotransferase activity; sulfotransferase activity; HNK-1 sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; 4-phenanthrol sulfotransferase activity; heparan sulfate 2-O-sulfotransferase activity; cholesterol sulfotransferase activity; trans-9R,10R-dihydrodiolphenanthrene sulfotransferase activity; N-acetylgalactosamine 4-O-sulfotransferase activity; 9-phenanthrol sulfotransferase activity; heparan sulfate 6-O-sulfotransferase activity

Biological Process: sulfur metabolic process; N-acetylglucosamine metabolic process; carbohydrate metabolic process; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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