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CILP elisa kit :: Human Cartilage intermediate layer protein 1 ELISA Kit

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Catalog # MBS2887177
Unit / Price
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cartilage intermediate layer protein 1 (CILP), ELISA Kit

Popular Item
Also Known As

Human Cartilage intermediate layer protein 1 ELISA Kit

Product Synonym Names
Cartilage intermediate layer protein 1; CILP-1; Cartilage intermediate-layer protein; CILP; UNQ602/PRO1188
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2887177 Testing Data
Sequence Length
1184
OMIM
603489
3D Structure
ModBase 3D Structure for O75339
Species Reactivity
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CILP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCILPpurchase
MBS2887177 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cartilage intermediate layer protein 1 (CILP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CILP. The ELISA analytical biochemical technique of the MBS2887177 kit is based on CILP antibody-CILP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CILP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CILP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CILP elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CILP elisa kit
CILP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2887177. Click to request current manual
NCBI/Uniprot data below describe general gene information for CILP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
132,565 Da
NCBI Official Full Name
cartilage intermediate layer protein 1 preproprotein
NCBI Official Synonym Full Names
cartilage intermediate layer protein
NCBI Official Symbol
NCBI Official Synonym Symbols
CILP-1; HsT18872
  [Similar Products]
NCBI Protein Information
cartilage intermediate layer protein 1
UniProt Protein Name
Cartilage intermediate layer protein 1
UniProt Synonym Protein Names
Cartilage intermediate-layer protein
UniProt Gene Name
UniProt Synonym Gene Names
CILP-1  [Similar Products]
UniProt Entry Name
CILP1_HUMAN
NCBI Summary for CILP
Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]
UniProt Comments for CILP
CILP: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Defects in CILP are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Ile395Thr (PubMed:15864306).

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: alkaline phosphatase activity; nucleotide diphosphatase activity

Biological Process: negative regulation of insulin-like growth factor receptor signaling pathway

Disease: Intervertebral Disc Disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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