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CKAP5 elisa kit :: Human Cytoskeleton associated protein 5 (CKAP5) ELISA Kit

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Catalog # MBS7251404
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cytoskeleton associated protein 5 (CKAP5), ELISA Kit

Popular Item
Full Product Name

Human Cytoskeleton associated protein 5 (CKAP5) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
This assay has high sensitivity and excellent specificity for detection of CKAP5. No significant cross-reactivity or interference between CKAP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CKAP5 and all the analogues, therefore, cross reaction may still exist in some cases.
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Detection Range
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CKAP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCKAP5purchase
MBS7251404 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytoskeleton associated protein 5 (CKAP5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CKAP5. The ELISA analytical biochemical technique of the MBS7251404 kit is based on CKAP5 antibody-CKAP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CKAP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CKAP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CKAP5 elisa kit
Intended Uses: This CKAP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CKAP5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CKAP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CKAP5 antibody and an CKAP5-HRP conjugate. The assay sample and buffer are incubated together with CKAP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKAP5 concentration since CKAP5 from samples and CKAP5-HRP conjugate compete for the anti-CKAP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CKAP5 from the sample, fewer sites are left to bind CKAP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKAP5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CKAP5 elisa kit

Typical Testing Data/Standard Curve (for reference only) of CKAP5 elisa kit
CKAP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7251404. Click to request current manual
NCBI/Uniprot data below describe general gene information for CKAP5. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
226,258 Da
NCBI Official Full Name
cytoskeleton associated protein 5
NCBI Official Synonym Full Names
cytoskeleton associated protein 5
NCBI Official Symbol
CKAP5  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
cytoskeleton-associated protein 5; colonic and hepatic tumor overexpressed gene protein; colonic and hepatic tumor over-expressed gene protein
UniProt Protein Name
Cytoskeleton-associated protein 5
UniProt Synonym Protein Names
Colonic and hepatic tumor overexpressed gene protein; Ch-TOG
UniProt Gene Name
CKAP5  [Similar Products]
UniProt Synonym Gene Names
KIAA0097; Ch-TOG  [Similar Products]
UniProt Entry Name
NCBI Summary for CKAP5
This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
UniProt Comments for CKAP5
CKAP5: Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. Belongs to the TOG/XMAP215 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: microtubule cytoskeleton; spindle pole; centrosome; protein complex; membrane; cytosol; gamma-tubulin complex

Molecular Function: protein binding

Biological Process: mitosis; centrosome organization and biogenesis; cell division; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; spindle organization and biogenesis; RNA transport; establishment and/or maintenance of microtubule cytoskeleton polarity
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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