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CKMB elisa kit :: Human Creatine Kinase MB Isoenzyme (CKMB) ELISA Kit

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Catalog # MBS265355
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Creatine Kinase MB Isoenzyme (CKMB), ELISA Kit

Popular Item
Also Known As

Human Creatine Kinase MB Isoenzyme (CKMB) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
381
OMIM
123310
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant CKMB concentration.
Assay Type
Sandwich
Detection Range
100 ng/ml-1.56 ng/ml
Sensitivity
0.5 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CKMB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCKMBpurchase
MBS265355 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine Kinase MB Isoenzyme (CKMB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CKMB. The ELISA analytical biochemical technique of the MBS265355 kit is based on CKMB antibody-CKMB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CKMB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CKMB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CKMB elisa kit
Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CKMB monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve of CKMB elisa kit
CKMB elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS265355. Click to request current manual
NCBI/Uniprot data below describe general gene information for CKMB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,101 Da
NCBI Official Full Name
creatine kinase M-type
NCBI Official Synonym Full Names
creatine kinase, muscle
NCBI Official Symbol
NCBI Official Synonym Symbols
CKMM; M-CK
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NCBI Protein Information
creatine kinase M-type; creatine kinase M chain
UniProt Protein Name
Creatine kinase M-type
UniProt Synonym Protein Names
Creatine kinase M chain; M-CKCleaved into the following chain:Creatine kinase M-type, N-terminally processed
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
KCRM_HUMAN
NCBI Summary for CKMB
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
UniProt Comments for CKMB
CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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