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CLDN18 elisa kit :: Rabbit Claudin-18 (CLDN18) ELISA Kit

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Catalog # MBS7204960
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Claudin-18 (CLDN18), ELISA Kit

Popular Item
Also Known As

Rabbit Claudin-18 (CLDN18) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
609210
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CLDN-18. No significant cross-reactivity or interference between CLDN-18 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN-18 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CLDN18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCLDN18purchase
MBS7204960 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Claudin-18 (CLDN18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLDN18. The ELISA analytical biochemical technique of the MBS7204960 kit is based on CLDN18 antibody-CLDN18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLDN18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLDN18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CLDN18 elisa kit
Intended Uses: This CLDN-18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CLDN-18. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CLDN-18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLDN-18 antibody and an CLDN-18-HRP conjugate. The assay sample and buffer are incubated together with CLDN-18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN-18 concentration since CLDN-18 from samples and CLDN-18-HRP conjugate compete for the anti-CLDN-18 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN-18 from the sample, fewer sites are left to bind CLDN-18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN-18 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLDN18 elisa kit

Typical Testing Data/Standard Curve (for reference only) of CLDN18 elisa kit
CLDN18 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7204960. Click to request current manual
NCBI/Uniprot data below describe general gene information for CLDN18. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,720 Da[Similar Products]
NCBI Official Full Name
claudin-18 isoform 2
NCBI Official Synonym Full Names
claudin 18
NCBI Official Symbol
CLDN18  [Similar Products]
NCBI Official Synonym Symbols
SFTA5; SFTPJ
  [Similar Products]
NCBI Protein Information
claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
UniProt Protein Name
Claudin-18
Protein Family
UniProt Gene Name
CLDN18  [Similar Products]
UniProt Synonym Gene Names
UNQ778/PRO1572  [Similar Products]
UniProt Entry Name
CLD18_HUMAN
NCBI Summary for CLDN18
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]
UniProt Comments for CLDN18
Claudin-18: a multi-pass membrane protein with calcium-independent cell-adhesion activity that plays a major role in the formation of tight junctions. Belongs to the claudin family. Two isoforms of the human protein are produced by alternative splicing. Isoform 2 is a specific cell lineage marker. Its expression in normal tissues is restricted to differentiated epithelial cells of the gastric mucosa; it is absent from the gastric stem cells. Isoform 2 is expressed in a significant proportion of primary gastric cancers and metastases, as well as in subtypes of pancreatic, esophageal, ovarian, and lung tumors.

Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; calcium-independent cell-cell adhesion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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