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CLDN5 elisa kit :: Mouse Claudin 5 (CLDN5) ELISA Kit

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Catalog # MBS456204
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $545 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,480 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,820 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Claudin 5 (CLDN5), ELISA Kit

Full Product Name

Mouse Claudin 5 (CLDN5) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for O54942
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CLDN5. No significant cross-reactivity or interference between CLDN5 and analogues was observed.
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.127ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLDN5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLDN5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CLDN5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CLDN5purchase
MBS456204 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Claudin 5 (CLDN5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLDN5. The ELISA analytical biochemical technique of the MBS456204 kit is based on CLDN5 antibody-CLDN5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLDN5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLDN5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CLDN5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CLDN5 in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLDN5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CLDN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLDN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLDN5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CLDN5 elisa kit
CLDN5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS456204. Click to request current manual
NCBI/Uniprot data below describe general gene information for CLDN5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,054 Da
NCBI Official Full Name
claudin-5
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
Cldn5  [Similar Products]
NCBI Official Synonym Symbols
MBEC1; Tmvcf; AI854493
  [Similar Products]
NCBI Protein Information
claudin-5
UniProt Protein Name
Claudin-5
UniProt Synonym Protein Names
Brain endothelial cell clone 1 protein; Lung-specific membrane protein
Protein Family
UniProt Gene Name
Cldn5  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for CLDN5
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a critical component of endothelial tight junctions that control pericellular permeability. The knockout mice lacking this gene died within 10 h of birth and the blood-brain barrier in these mice against small molecules was selectively affected. This gene is expressed strongly in endothelium of normal lung and plays a regulation role during acrolein-induced acute lung injury. [provided by RefSeq, Aug 2010]
UniProt Comments for CLDN5
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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