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CLEC1A elisa kit :: Human C type lectin domain family 1 member A (CLEC1A) ELISA Kit

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Catalog # MBS7236626
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

C type lectin domain family 1 member A (CLEC1A), ELISA Kit

Popular Item
Also Known As

Human C type lectin domain family 1 member A (CLEC1A) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
606782
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CLEC1A. No significant cross-reactivity or interference between CLEC1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC1A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CLEC1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCLEC1Apurchase
MBS7236626 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C type lectin domain family 1 member A (CLEC1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLEC1A. The ELISA analytical biochemical technique of the MBS7236626 kit is based on CLEC1A antibody-CLEC1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLEC1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLEC1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CLEC1A elisa kit
Intended Uses: This CLEC1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CLEC1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC1A antibody and an CLEC1A-HRP conjugate. The assay sample and buffer are incubated together with CLEC1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC1A concentration since CLEC1A from samples and CLEC1A-HRP conjugate compete for the anti-CLEC1A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC1A from the sample, fewer sites are left to bind CLEC1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC1A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLEC1A elisa kit

Typical Testing Data/Standard Curve (for reference only) of CLEC1A elisa kit
CLEC1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7236626. Click to request current manual
NCBI/Uniprot data below describe general gene information for CLEC1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,952 Da
NCBI Official Full Name
C-type lectin domain family 1 member A
NCBI Official Synonym Full Names
C-type lectin domain family 1, member A
NCBI Official Symbol
CLEC1A  [Similar Products]
NCBI Official Synonym Symbols
CLEC1; CLEC-1
  [Similar Products]
NCBI Protein Information
C-type lectin domain family 1 member A; C-type lectin-like receptor-1
UniProt Protein Name
C-type lectin domain family 1 member A
UniProt Synonym Protein Names
C-type lectin-like receptor 1; CLEC-1
UniProt Gene Name
CLEC1A  [Similar Products]
UniProt Synonym Gene Names
CLEC1; UNQ569/PRO1131; CLEC-1  [Similar Products]
UniProt Entry Name
CLC1A_HUMAN
NCBI Summary for CLEC1A
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. The encoded protein may play a role in regulating dendritic cell function. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for CLEC1A
CLEC1A:

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: integral to plasma membrane; intracellular

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction; defense response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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