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CLEC2D elisa kit :: Human C-type lectin domain family 2, member D ELISA Kit

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Catalog # MBS936829
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Typical Testing Data/Standard Curve (for reference only)
Product Name

C-type lectin domain family 2, member D (CLEC2D), ELISA Kit

Popular Item
Full Product Name

Human C-type lectin domain family 2 member D, CLEC2D ELISA Kit

Product Synonym Names
Human C-type lectin domain family 2 member D (CLEC2D) ELISA kit; CLAX; LLT1; OCIL; C-type lectin related f; C-type lectin superfamily 2; member D; lectin-like NK cell receptor; lectin-like transcript 1; osteoclast inhibitory lectin; C-type lectin domain family 2; member D
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605659
3D Structure
ModBase 3D Structure for Q9UHP7
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CLEC2D. No significant cross-reactivity or interference between human CLEC2D and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich (Quantitative)
Detection Range
31.25 pg/ml-2000 pg/ml.
Sensitivity
7.81 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CLEC2D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CLEC2Dpurchase
MBS936829 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-type lectin domain family 2, member D (CLEC2D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLEC2D. The ELISA analytical biochemical technique of the MBS936829 kit is based on CLEC2D antibody-CLEC2D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLEC2D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLEC2D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CLEC2D elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLEC2D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLEC2D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLEC2D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLEC2D bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CLEC2D elisa kit
CLEC2D elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for CLEC2D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,849 Da
NCBI Official Full Name
C-type lectin domain family 2 member D isoform 2
NCBI Official Synonym Full Names
C-type lectin domain family 2, member D
NCBI Official Symbol
CLEC2D  [Similar Products]
NCBI Official Synonym Symbols
CLAX; LLT1; OCIL
  [Similar Products]
NCBI Protein Information
C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, member D
UniProt Protein Name
C-type lectin domain family 2 member D
UniProt Synonym Protein Names
Lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; Osteoclast inhibitory lectin
UniProt Gene Name
CLEC2D  [Similar Products]
UniProt Synonym Gene Names
CLAX; LLT1; OCIL; LLT-1  [Similar Products]
UniProt Entry Name
CLC2D_HUMAN
NCBI Summary for CLEC2D
This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Oct 2010]
UniProt Comments for CLEC2D
CLEC2D: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon- gamma. Binds high molecular weight sulfated glycosaminoglycans. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cell surface; membrane; integral to plasma membrane; endoplasmic reticulum

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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