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CLIA Kits

Many customers may ask: when should I select a CLIA assay?

Let's start with the basics, the underlying immunoassay principles. Enzyme immunoassay (EIA) in broad terms is a biochemical immunoassay that utilizes an enzyme labeled antibody or antigen to detect a target antibody or antigen. The target is also referred as the analyte and the antigens being detected are often proteins.

Enzyme-linked immunosorbent assay (ELISA), first reported in 1971, is the most common EIA detection method. It is also among the most widely used bioanalytical methods for measuring the concentration of an analyte in solution. In ELISA, the analyte is typically detected through an observable color change generated by a chromogenic reporter and substrate.

An increasing need to detect ever-smaller amounts of target analyte molecules in liquids or wet samples led to the emergence of the chemiluminescence immunoassay (CLIA), first reported in 1981. CLIAs increased the analytical sensitivity of immunoassays by using chemiluminescent indicators such as luminol to directly label the detection antibody or antigen.

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