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CLIC1 elisa kit :: Mouse chloride intracellular channel 1 ELISA Kit

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Catalog # MBS926340
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Typical Testing Data/Standard Curve (for reference only)
Product Name

chloride intracellular channel 1 (CLIC1), ELISA Kit

Popular Item
Also Known As

Mouse Chloride intracellular channel protein 1, CLIC1 ELISA Kit

Product Synonym Names
Mouse Chloride intracellular channel protein 1 (CLIC1) ELISA kit; DADB-110M10.1; G6; NCC27; OTTHUMP00000029133; OTTHUMP00000029137; OTTHUMP00000174486; RNCC protein; chloride channel ABP; nuclear chloride ion channel protein; p64CLCP; chloride intracellular channel 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS926340 Testing Data
Sequence Length
241
3D Structure
ModBase 3D Structure for Q9Z1Q5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CLIC1. No significant cross-reactivity or interference between mouse CLIC1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
12.5 pg/ml -800 pg/ml.
Sensitivity
The minimum detectable dose of mouse CLIC1 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CLIC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCLIC1purchase
MBS926340 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chloride intracellular channel 1 (CLIC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLIC1. The ELISA analytical biochemical technique of the MBS926340 kit is based on CLIC1 antibody-CLIC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLIC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLIC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CLIC1 elisa kit
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLIC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLIC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLIC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLIC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CLIC1 elisa kit
CLIC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS926340. Click to request current manual
NCBI/Uniprot data below describe general gene information for CLIC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
27,013 Da
NCBI Official Full Name
chloride intracellular channel protein 1
NCBI Official Synonym Full Names
chloride intracellular channel 1
NCBI Official Symbol
Clic1  [Similar Products]
NCBI Official Synonym Symbols
G6; Clcp
  [Similar Products]
NCBI Protein Information
chloride intracellular channel protein 1; NCC27; nuclear chloride ion channel 27
UniProt Protein Name
Chloride intracellular channel protein 1
UniProt Synonym Protein Names
Nuclear chloride ion channel 27; NCC27
UniProt Gene Name
Clic1  [Similar Products]
UniProt Synonym Gene Names
NCC27  [Similar Products]
UniProt Entry Name
CLIC1_MOUSE
UniProt Comments for CLIC1
CLIC1: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle. Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral; Channel, chloride

Cellular Component: extracellular space; mitochondrion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane; nuclear envelope; nucleus; vesicle

Molecular Function: chloride channel activity; voltage-gated ion channel activity

Biological Process: positive regulation of osteoblast differentiation; regulation of mitochondrial membrane potential; regulation of cell cycle; transport; ion transport; chloride transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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