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CLU elisa kit :: Mouse Clusterin ELISA Kit

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Catalog # MBS176507
Unit / Price
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  96-Strip-Wells  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Clusterin, ELISA Kit

Popular Item
Full Product Name

Mouse Clusterin PicoKine ELISA Kit

Product Synonym Names
Clusterin; clusterin; AAG4; Aging associated protein 4; APO J; APOJ; CLI; CLU; CLU1; CLU2; Clusterin; Complement associated protein SP 40,40; Complement cytolysis inhibitor; Complement lysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; SP40; Sulfated glycoprotein 2; Testosterone repressed prostate message 2; TRPM 2; TRPM2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for Q06890
Species Reactivity
Specificity
Natural and recombinant mouse Clusterin
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
312pg/ml-20,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CLU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CLUpurchase
MBS176507 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Clusterin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLU. The ELISA analytical biochemical technique of the MBS176507 kit is based on CLU antibody-CLU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CLU elisa kit
Principle of the assay: mouse Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Clusterin has been precoated onto 96-well plates. Standards(NSO, D22-R226(beta)&S227-E448(alpha)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Clusterin amount of sample captured in plate.
Background: Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14.Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death.In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Typical Testing Data/Standard Curve (for reference only) of CLU elisa kit
Mouse Clusterin PicoKine ELISA Kit standard curve
CLU elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for CLU. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
51,656 Da
NCBI Official Full Name
Clusterin
NCBI Official Synonym Full Names
clusterin
NCBI Official Symbol
NCBI Official Synonym Symbols
Cli; ApoJ; Sgp2; SP-40; Sgp-2; Sugp-2; AI893575; D14Ucla3
  [Similar Products]
NCBI Protein Information
clusterin; apo-J; clustrin; Apolipoprotein J; sulfated glycoprotein 2; complement lysis inhibitor; testosterone repressed prostate message; testosterone repressed prostate message-2
UniProt Protein Name
Clusterin
UniProt Synonym Protein Names
Apolipoprotein J; Apo-J; Clustrin; Sulfated glycoprotein 2; SGP-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Apoj; Msgp-2; Apo-J; SGP-2  [Similar Products]
UniProt Entry Name
CLUS_MOUSE
NCBI Summary for CLU
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. [provided by RefSeq, May 2011]
UniProt Comments for CLU
CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Apoptosis; Secreted; Mitochondrial

Cellular Component: extracellular matrix; extracellular space; neuron projection; growth cone; intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; protein N-terminus binding; misfolded protein binding

Biological Process: cell death; response to misfolded protein; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; endocrine pancreas development; activation of NF-kappaB transcription factor; negative regulation of protein homooligomerization; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; positive regulation of cell proliferation; chaperone-mediated protein complex assembly; positive regulation of cell differentiation; neurite morphogenesis; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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