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Col II elisa kit :: Rat Collagen Type II ELISA Kit

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Catalog # MBS728101
Unit / Price
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  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Collagen Type II (Col II), ELISA Kit

Popular Item
Full Product Name

Rat Collagen Type II ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
609508
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Col II elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCol IIpurchase
MBS728101 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type II (Col II) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Col II. The ELISA analytical biochemical technique of the MBS728101 kit is based on Col II antibody-Col II antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Col II antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Col II. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Col II elisa kit
Intended Uses: This CoL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CoL-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CoL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CoL-2 antibody and an CoL-2-HRP conjugate. The assay sample and buffer are incubated together with CoL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CoL-2 concentration since CoL-2 from samples and CoL-2-HRP conjugate compete for the anti-CoL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CoL-2 from the sample, fewer sites are left to bind CoL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Col II elisa kit

Typical Testing Data/Standard Curve (for reference only) of Col II elisa kit
Col II elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS728101. Click to request current manual
NCBI/Uniprot data below describe general gene information for Col II. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,781 Da
NCBI Official Full Name
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), isoform CRA_e
NCBI Official Synonym Full Names
collagen, type II, alpha 1
NCBI Official Symbol
COL2A1  [Similar Products]
NCBI Official Synonym Symbols
AOM; ANFH; SEDC; STL1; COL11A3
  [Similar Products]
NCBI Protein Information
collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; arthroophthalmopathy, progressive (Stickler syndrome)
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Synonym Protein Names
Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin
UniProt Gene Name
COL2A1  [Similar Products]
UniProt Entry Name
CO2A1_HUMAN
NCBI Summary for Col II
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for Col II
COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type II; extracellular region; basement membrane

Molecular Function: identical protein binding; platelet-derived growth factor binding; metal ion binding; extracellular matrix structural constituent conferring tensile strength

Biological Process: heart morphogenesis; axon guidance; proteoglycan metabolic process; extracellular matrix organization and biogenesis; inner ear morphogenesis; collagen fibril organization; central nervous system development; palate development; notochord development; extracellular matrix disassembly; limb bud formation; collagen catabolic process; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; cartilage condensation; endochondral ossification

Disease: Achondrogenesis, Type Ii; Kniest Dysplasia; Legg-calve-perthes Disease; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Spondyloperipheral Dysplasia; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Avascular Necrosis Of Femoral Head, Primary; Spondyloepiphyseal Dysplasia Congenita; Stickler Syndrome, Type I, Nonsyndromic Ocular
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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