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COL15 elisa kit :: Human Collagen Type XV (COL15) ELISA Kit

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Catalog # MBS2704218 (SPECIAL PROMOTIONAL PRICING for a limited time)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Collagen Type XV (COL15), ELISA Kit

Popular Item
Full Product Name

Human Collagen Type XV (COL15) ELISA Kit

Product Synonym Names
COL15A1; COL15-A1; Collagen Alpha-1(XV)chain; Restin; Endostatin-XV; Related to endostatin;
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
L25286 mRNA
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Collagen Type XV (COL15).
No significant cross-reactivity or interference between Collagen Type XV (COL15) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates and Other Biological Fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 1.33ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Collagen Type XV (COL15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Collagen Type XV (COL15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COL15 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for COL15purchase
MBS2704218 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type XV (COL15) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL15. The ELISA analytical biochemical technique of the MBS2704218 kit is based on COL15 antibody-COL15 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL15 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL15. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
COL15 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XV (COL15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XV (COL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XV (COL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type XV (COL15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of COL15 elisa kit
COL15 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2704218. Click to request current manual
NCBI/Uniprot data below describe general gene information for COL15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
141,720 Da
NCBI Official Full Name
collagen, type XV, alpha 1, isoform CRA_f
NCBI Official Synonym Full Names
collagen type XV alpha 1 chain
NCBI Official Symbol
COL15A1  [Similar Products]
NCBI Protein Information
collagen alpha-1(XV) chain
UniProt Protein Name
Collagen alpha-1(XV) chain
UniProt Synonym Protein Names
RestinAlternative name(s):Endostatin-XV; Related to endostatin; Restin-I
UniProt Gene Name
COL15A1  [Similar Products]
NCBI Summary for COL15
This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration. [provided by RefSeq, May 2013]
UniProt Comments for COL15
COL15A1: Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle. Belongs to the multiplexin collagen family.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q22.33

Cellular Component: collagen type XV trimer; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space; integral component of membrane

Molecular Function: extracellular matrix structural constituent

Biological Process: angiogenesis; cell adhesion; cell differentiation; collagen catabolic process; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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