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COL18A1 elisa kit :: Human Collagen Alpha 1 (XVIII) chain (COL18A1) ELISA Kit

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Catalog # MBS7201077
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Collagen Alpha 1 (XVIII) chain (COL18A1), ELISA Kit

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Full Product Name

Human Collagen Alpha 1 (XVIII) chain (COL18A1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CoL-18A1. No significant cross-reactivity or interference between CoL-18A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CoL-18A1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This CoL-18A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CoL-18A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COL18A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCOL18A1purchase
MBS7201077 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Alpha 1 (XVIII) chain (COL18A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL18A1. The ELISA analytical biochemical technique of the MBS7201077 kit is based on COL18A1 antibody-COL18A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL18A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL18A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Besides normal localization to extracellular matrix as a chain component, Collagen alpha 1 type XVIII also has antiangiogenic signaling properties as an endostatin. Mutations of the COL18A1 gene result in retinal abnormalities in humans and thus research is centered on sight saving measures. Aiding this important work is a specific HRP - AMP based sandwich ELISA for XvIII collagen, with no cross reaction to other human collagen subtypes established. This ELISA kit is complete with all products required for analysis of cell proteins or bodily tissues. Rapid results and sensitivity range - 0.1 ng mL - have made these ELISA kits a favorite for human col and also for monkey ELISA kits or Mouse ELISA collagen type XVIII as well. Assay details, including antibodies (organism specific for kits ELISA), standard curve product, 96 tests plate and antibody info are packed with COL18A1 ELISA.
Related Product Information for
COL18A1 elisa kit
Principle of the assay: CoL-18A1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CoL-18A1. Standards or samples are then added to the microtiter plate wells and CoL-18A1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CoL-18A1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CoL-18A1 are added to each well to "sandwich" the CoL-18A1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CoL-18A1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-18A1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for COL18A1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of COL18A1 elisa kit
COL18A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7201077. Click to request current manual
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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