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COL2A1 elisa kit :: Rat collagen, type II, alpha 1 ELISA Kit

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Catalog # MBS907543
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Typical Testing Data/Standard Curve (for reference only)
Product Name

collagen, type II, alpha 1 (COL2A1), ELISA Kit

Full Product Name

Rat Collagen alpha-1 (II) chain, COL2A1 ELISA Kit

Product Synonym Names
Rat Collagen alpha-1 (II) chain (COL2A1) ELISA kit; ANFH; AOM; COL11A3; MGC131516; SEDC; cartilage collagen; chondrocalcin; collagen II; alpha-1 polypeptide; collagen; type II; alpha 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS907543 Testing Data
3D Structure
ModBase 3D Structure for P05539
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of rat COL2A1. No significant cross-reactivity or interference between rat COL2A1 and analogues was observed.
Serum, plasma, tissue homogenates
Assay Type
Detection Range
31.25 pg/ml -2000 pg/ml.
The minimum detectable dose of rat COL2A1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COL2A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCOL2A1purchase
MBS907543 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the collagen, type II, alpha 1 (COL2A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL2A1. The ELISA analytical biochemical technique of the MBS907543 kit is based on COL2A1 antibody-COL2A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL2A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL2A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
COL2A1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL2A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of COL2A1 elisa kit
COL2A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS907543. Click to request current manual
NCBI/Uniprot data below describe general gene information for COL2A1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
134,570 Da
NCBI Official Full Name
collagen alpha-1(II) chain
NCBI Official Synonym Full Names
collagen, type II, alpha 1
NCBI Official Symbol
Col2a1  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
collagen alpha-1(II) chain; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; procollagen, type II, alpha 1; collagen type II (Col2A1) gene, enhancer region
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Synonym Protein Names
Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin
UniProt Gene Name
Col2a1  [Similar Products]
UniProt Entry Name
NCBI Summary for COL2A1
structural component of the cartilage extracellular matrix [RGD, Feb 2006]
UniProt Comments for COL2A1
Function: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with COL2A1 elisa kit
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