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COL4A3BP elisa kit :: Mouse collagen, type IV, alpha 3 (Goodpasture antigen) binding protein ELISA Kit

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Catalog # MBS902024
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COL4A3BP elisa kit
Product Name

collagen, type IV, alpha 3 (Goodpasture antigen) binding protein (COL4A3BP), ELISA Kit

Full Product Name

Mouse Collagen type IV alpha-3-binding protein, COL4A3BP ELISA Kit

Product Synonym Names
Mouse Collagen type IV alpha-3-binding protein (COL4A3BP) ELISA kit; CERT; CERTL; FLJ20597; GPBP; STARD11; Goodpasture antigen-binding protein; OTTHUMP00000128248; START domain containing 11; StAR-related lipid transfer (START) domain containing 11; alpha 3 type IV colla; collagen; type IV; alpha 3 (Goodpasture antigen) binding protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9EQG9
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse COL4A3BP. No significant cross-reactivity or interference between Mouse COL4A3BP and analogues was observed.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transport
Protein Biological Process 3
Lipid transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COL4A3BP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for COL4A3BPpurchase
MBS902024 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the collagen, type IV, alpha 3 (Goodpasture antigen) binding protein (COL4A3BP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL4A3BP. The ELISA analytical biochemical technique of the MBS902024 kit is based on COL4A3BP antibody-COL4A3BP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL4A3BP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL4A3BP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for COL4A3BP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
71,111 Da
NCBI Official Full Name
collagen type IV alpha-3-binding protein isoform 2
NCBI Official Synonym Full Names
collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
NCBI Official Symbol
Col4a3bp  [Similar Products]
NCBI Official Synonym Symbols
CERT; GPBP; AU016711; 2810404O15Rik; 9230101K08Rik
  [Similar Products]
NCBI Protein Information
collagen type IV alpha-3-binding protein; stARD11; ceramide transfer protein; ceramide transport protein; START domain-containing protein 11; goodpasture antigen-binding protein; stAR-related lipid transfer protein 11; procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein
UniProt Protein Name
Collagen type IV alpha-3-binding protein
UniProt Synonym Protein Names
Ceramide transfer protein; Goodpasture antigen-binding protein; GPBP; START domain-containing protein 11; StARD11; StAR-related lipid transfer protein 11
UniProt Gene Name
Col4a3bp  [Similar Products]
UniProt Synonym Gene Names
Stard11; GPBP; StARD11  [Similar Products]
UniProt Entry Name
C43BP_MOUSE
UniProt Comments for COL4A3BP
COL4A3BP: a protein with a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. Implicated in human autoimmune diseases via its association with the Goodpasture antigen. Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. GPBP has been reported to have Ser/Thr protein kinase activity towards the Goodpasture (GP) antigen, but lacks a conventional serine/threonine kinase domain. Two human isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Lipid-binding; Protein kinase, atypical; Kinase, protein; EC 2.7.11.9; ATYPICAL group; COL4A3BP family

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: kinase activity; lipid binding

Biological Process: endoplasmic reticulum organization and biogenesis; heart morphogenesis; cell proliferation; muscle contraction; in utero embryonic development; transport; cell morphogenesis; lipid homeostasis; signal transduction; lipid transport; ceramide metabolic process; phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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