• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

COL6A3 elisa kit :: Human Collagen Alpha 3 (VI) chain (COL6A3) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS7241376
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Collagen Alpha 3 (VI) chain (COL6A3), ELISA Kit

Popular Item
Full Product Name

Human Collagen Alpha 3 (VI) chain (COL6A3) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CoL6alpha3. No significant cross-reactivity or interference between CoL6alpha3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CoL6alpha3 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COL6A3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for COL6A3purchase
MBS7241376 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Alpha 3 (VI) chain (COL6A3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL6A3. The ELISA analytical biochemical technique of the MBS7241376 kit is based on COL6A3 antibody-COL6A3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL6A3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL6A3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
COL6A3 elisa kit
Intended Uses: This CoL6alpha3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CoL6alpha3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CoL6alpha3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CoL6alpha3 antibody and an CoL6alpha3-HRP conjugate. The assay sample and buffer are incubated together with CoL6alpha3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CoL6alpha3 concentration since CoL6alpha3 from samples and CoL6alpha3-HRP conjugate compete for the anti-CoL6alpha3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CoL6alpha3 from the sample, fewer sites are left to bind CoL6alpha3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL6alpha3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for COL6A3 elisa kit

Typical Testing Data/Standard Curve of COL6A3 elisa kit
COL6A3 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS7241376. Click to request current manual
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000