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COLEC11 recombinant protein :: Collectin 11 Recombinant Protein

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Catalog # MBS638161
Unit / Price
  0.05 mg  /  $710 +1 FREE 8GB USB
COLEC11 recombinant protein
Product Name

Collectin 11 (COLEC11), Recombinant Protein

Popular Item
Also Known As

Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (3642422..3692234). Location: 2p25.3
OMIM
265050
3D Structure
ModBase 3D Structure for Q9BWP8
Purity/Purification
>85% as determined by SDS-PAGE under reducing conditions and visualized by silver strain.
Form/Format
Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS to a concentration of 0.1mg/ml.
Activity
Measured by its ability to bind Alpha-L-Fucose.
Endotoxin Level
<1EU per 1ug of protein (LAL)
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of COLEC11 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
COLEC11 recombinant protein
Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1-3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37kD collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23%-32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

DNA sequence encoding mouse CL-K1 (Gln 27-Leu 272; Accession # AAI0438) was fused to a 6x polyhistidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Applications Tested/Suitable for COLEC11 recombinant protein
Western Blot (WB)
Other applications not tested
Application Notes for COLEC11 recombinant protein
Dilution: Western Blot: As a result of glycosylation the recombinant mouse CL-K1 migrates as an ~33-38kD protein in SDS-PAGE under reducing conditions.
Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for COLEC11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
collectin-11 isoform a
NCBI Official Synonym Full Names
collectin sub-family member 11
NCBI Official Symbol
COLEC11  [Similar Products]
NCBI Official Synonym Symbols
3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
  [Similar Products]
NCBI Protein Information
collectin-11; collectin kidney protein 1
UniProt Protein Name
Collectin-11
UniProt Synonym Protein Names
Collectin kidney protein 1
Protein Family
UniProt Gene Name
COLEC11  [Similar Products]
UniProt Synonym Gene Names
CL-K1  [Similar Products]
UniProt Entry Name
COL11_HUMAN
NCBI Summary for COLEC11
This gene encodes a member of the collectin family of C-type lectins that possess collagen-like sequences and carbohydrate recognition domains. Collectins are secreted proteins that play important roles in the innate immune system by binding to carbohydrate antigens on microorganisms, facilitating their recognition and removal. The encoded protein binds to multiple sugars with a preference for fucose and mannose. Mutations in this gene are a cause of 3MC syndrome-2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Comments for COLEC11
COLEC11: Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N- acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration. Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2). 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the COLEC10/COLEC11 family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: collagen; extracellular region

Molecular Function: mannose binding

Biological Process: receptor-mediated endocytosis; multicellular organismal development; developmental process

Disease: 3mc Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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