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COLEC12 elisa kit :: Human collectin sub-family member 12 ELISA Kit

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Catalog # MBS907179
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

collectin sub-family member 12 (COLEC12), ELISA Kit

Popular Item
Full Product Name

Human Collectin-12, COLEC12 ELISA Kit

Product Synonym Names
Human Collectin-12 (COLEC12) ELISA kit; CLP1; NSR2; SCARA4; SRCL; collectin placenta 1; nurse cell scavenger receptor 2; scavenger receptor class A; member 4; scavenger receptor with C-type lectin; collectin sub-family member 12
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS907179 Testing Data
OMIM
607621
3D Structure
ModBase 3D Structure for Q5KU26
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human COLEC12. No significant cross-reactivity or interference between human COLEC12 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 ng/ml -20 ng/mL
Sensitivity
<0.078 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COLEC12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for COLEC12purchase
MBS907179 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the collectin sub-family member 12 (COLEC12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COLEC12. The ELISA analytical biochemical technique of the MBS907179 kit is based on COLEC12 antibody-COLEC12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COLEC12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COLEC12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
COLEC12 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COLEC12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COLEC12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COLEC12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COLEC12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of COLEC12 elisa kit
COLEC12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS907179. Click to request current manual
NCBI/Uniprot data below describe general gene information for COLEC12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,515 Da
NCBI Official Full Name
collectin-12
NCBI Official Synonym Full Names
collectin sub-family member 12
NCBI Official Symbol
COLEC12  [Similar Products]
NCBI Official Synonym Symbols
CLP1; NSR2; SRCL; SCARA4
  [Similar Products]
NCBI Protein Information
collectin-12; hCL-P1; collectin placenta protein 1; nurse cell scavenger receptor 2; scavenger receptor class A, member 4; scavenger receptor with C-type lectin
UniProt Protein Name
Collectin-12
UniProt Synonym Protein Names
Collectin placenta protein 1; CL-P1; hCL-P1; Nurse cell scavenger receptor 2; Scavenger receptor class A member 4; Scavenger receptor with C-type lectin
Protein Family
UniProt Gene Name
COLEC12  [Similar Products]
UniProt Synonym Gene Names
CLP1; NSR2; SCARA4; SRCL; CL-P1; hCL-P1  [Similar Products]
UniProt Entry Name
COL12_HUMAN
NCBI Summary for COLEC12
This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. This protein is a scavenger receptor, a cell surface glycoprotein that displays several functions associated with host defense. It can bind to carbohydrate antigens on microorganisms, facilitating their recognition and removal. It also mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein by vascular endothelial cells. [provided by RefSeq, Oct 2011]
UniProt Comments for COLEC12
COLEC12: Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid beta in Alzheimer disease.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: collagen; integral to membrane; plasma membrane

Molecular Function: metal ion binding; galactose binding; low-density lipoprotein binding; pattern recognition receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; innate immune response; carbohydrate mediated signaling; phagocytosis, recognition; defense response; pattern recognition receptor signaling pathway; protein homooligomerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with COLEC12 elisa kit
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