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COMP elisa kit :: Human COMP ELISA Kit

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Catalog # MBS824705
Unit / Price
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  96 Tests  /  $470 +1 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

COMP, ELISA Kit

Popular Item
Full Product Name

Human COMP ELISA Kit

Product Synonym Names
Cartilage oligomeric matrix protein; COMP; Thrombospondin-5; TSP5
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
phenotype 600310
3D Structure
ModBase 3D Structure for P49747
Species Reactivity
Specificity
The Human COMP ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human COMP proteins within the range of 156 pg/ml - 10000 pg/ml.
Samples
Serum, plasma
Sensitivity
Typically less than 10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COMP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for COMPpurchase
MBS824705 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COMP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COMP. The ELISA analytical biochemical technique of the MBS824705 kit is based on COMP antibody-COMP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COMP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COMP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
COMP elisa kit
Principle of the Assay: The Human COMP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human COMP in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human COMP coated on a 96-well plate. Standards and samples are pipetted into the wells and COMP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human COMP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COMP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
Applications Tested/Suitable for COMP elisa kit
Sandwich ELISA (SE)

Typical Standard Curve/Testing Data of COMP elisa kit
COMP elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS824705. Click to request current manual
NCBI/Uniprot data below describe general gene information for COMP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
77,214 Da
NCBI Official Full Name
cartilage oligomeric matrix protein
NCBI Official Synonym Full Names
cartilage oligomeric matrix protein
NCBI Official Symbol
NCBI Official Synonym Symbols
MED; EDM1; EPD1; PSACH; THBS5
  [Similar Products]
NCBI Protein Information
cartilage oligomeric matrix protein; TSP5; thrombospondin-5; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)
UniProt Protein Name
Cartilage oligomeric matrix protein
UniProt Synonym Protein Names
Thrombospondin-5; TSP5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
COMP; TSP5  [Similar Products]
UniProt Entry Name
COMP_HUMAN
NCBI Summary for COMP
The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]
UniProt Comments for COMP
COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; collagen binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: limb development; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis

Disease: Pseudoachondroplasia; Epiphyseal Dysplasia, Multiple, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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