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COMT recombinant protein

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Catalog # MBS203262
Unit / Price
  0.1 mg  /  $510 +1 FREE 8GB USB
  0.5 mg  /  $1,335 +1 FREE 8GB USB
SDS-Page
Product Name

COMT, Recombinant Protein

Popular Item
Also Known As

COMT, 51-271aa, Human, E Coli

Product Synonym Names
Catechol-O-methyltransferase; Catechol-O-methyltransferase; Catechol-O-methyltransferase; COMT
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
271
Sequence
MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P
OMIM
M65212 mRNA
3D Structure
ModBase 3D Structure for P21964
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM MgCl2, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of COMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
COMT recombinant protein
Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for COMT recombinant protein
Applications Tested/Suitable for COMT recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for COMT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24.4 kDa (221 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
catechol O-methyltransferase isoform MB-COMT
NCBI Official Synonym Full Names
catechol-O-methyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
HEL-S-98n
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NCBI Protein Information
catechol O-methyltransferase
UniProt Protein Name
Catechol O-methyltransferase
UniProt Gene Name
NCBI Summary for COMT
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
UniProt Comments for COMT
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Product References and Citations for COMT recombinant protein
Cotton NJ., et al. (2004) J Biol Chem. 279 (22):23710-8. ; Dawling S., et al. (2001) Cancer Res. 61 (18):6716-22.;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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