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Corin elisa kit :: Canine Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit

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Catalog # MBS753915
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Corin (Atrial natriuretic peptide-converting enzyme), ELISA Kit

Popular Item
Also Known As

Canine Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 4; NC_000004.11 (47596018..47840059, complement). Location: 4p13-p12
OMIM
605236
3D Structure
ModBase 3D Structure for Q9Y5Q5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Corin. No significant cross-reactivity or interference between Corin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Corin and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Corin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCorinpurchase
MBS753915 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Corin. The ELISA analytical biochemical technique of the MBS753915 kit is based on Corin antibody-Corin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Corin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Corin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Corin elisa kit
Intended Uses: This Corin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine Corin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: Corin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Corin antibody and an Corin-HRP conjugate. The assay sample and buffer are incubated together with Corin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Corin concentration since Corin from samples and Corin-HRP conjugate compete for the anti-Corin antibody binding site. Since the number of sites is limited, as more sites are occupied by Corin from the sample, fewer sites are left to bind Corin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Corin concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Corin elisa kit

Typical Testing Data/Standard Curve (for reference only) of Corin elisa kit
Corin elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS753915. Click to request current manual
NCBI/Uniprot data below describe general gene information for Corin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
116,486 Da
NCBI Official Full Name
corin
NCBI Official Synonym Full Names
corin, serine peptidase
NCBI Official Symbol
CORIN  [Similar Products]
NCBI Official Synonym Symbols
CRN; ATC2; Lrp4; PEE5; TMPRSS10
  [Similar Products]
NCBI Protein Information
atrial natriuretic peptide-converting enzyme; pro-ANP-convertase; pro-ANP-converting enzyme; transmembrane protease serine 10; heart-specific serine proteinase ATC2
UniProt Protein Name
Atrial natriuretic peptide-converting enzyme
UniProt Synonym Protein Names
Corin; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme; Transmembrane protease serine 10
UniProt Gene Name
CORIN  [Similar Products]
UniProt Synonym Gene Names
CRN; TMPRSS10  [Similar Products]
UniProt Entry Name
CORIN_HUMAN
NCBI Summary for Corin
This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
UniProt Comments for Corin
CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface; Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide

Disease: Preeclampsia/eclampsia 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withCorin elisa kit
 Reference Product  PubMed Publications
 NPPA elisa kit  >1 publications with Corin and NPPA
Pathways associated with Corin elisa kit
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