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COTININE elisa kit :: Human Cotinine ELISA Kit

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Catalog # MBS019457
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cotinine, ELISA Kit

Popular Item
Also Known As

Human Cotinine ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
239
OMIM
605608
3D Structure
ModBase 3D Structure for O95500
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
undiluted original Human body fluids, tissue homogenates, secretions or feces samples
Assay Type
Quantitative Sandwich
Detection Range
0.5 ng/ml - 16 ng/mL
Sensitivity
0.1 ng/mL
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COTININE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCOTININEpurchase
MBS019457 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cotinine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COTININE. The ELISA analytical biochemical technique of the MBS019457 kit is based on COTININE antibody-COTININE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COTININE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COTININE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
COTININE elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CTN (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

Typical Testing Data/Standard Curve (for reference only) of COTININE elisa kit
COTININE elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS019457. Click to request current manual
NCBI/Uniprot data below describe general gene information for COTININE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
25,699 Da
NCBI Official Full Name
claudin-14
NCBI Official Synonym Full Names
claudin 14
NCBI Official Symbol
CLDN14  [Similar Products]
NCBI Official Synonym Symbols
DFNB29
  [Similar Products]
NCBI Protein Information
claudin-14
UniProt Protein Name
Claudin-14
UniProt Gene Name
CLDN14  [Similar Products]
UniProt Entry Name
CLD14_HUMAN
NCBI Summary for COTININE
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. It is also reported that four synonymous variants in this gene are associated with kidney stones and reduced bone mineral density. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Comments for COTININE
Claudin-14: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Defects in CLDN14 are the cause of deafness autosomal recessive type 29 (DFNB29). DFNB29 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: tight junction; endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; protein complex assembly; calcium-independent cell-cell adhesion

Disease: Deafness, Autosomal Recessive 29
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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