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CPE elisa kit :: Mouse Carboxypeptidase E ELISA Kit

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Catalog # MBS281703
Unit / Price
  48-Strip-Wells  /  $405 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,080 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,805 +7 FREE 8GB USB
CPE elisa kit
Product Name

Carboxypeptidase E (CPE), ELISA Kit

Also Known As

Mouse Carboxypeptidase E (CPE) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
476
3D Structure
ModBase 3D Structure for Q00493
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CPE. No significant cross-reactivity or interference between Mouse CPE and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CPE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCPEpurchase
MBS281703 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carboxypeptidase E (CPE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CPE. The ELISA analytical biochemical technique of the MBS281703 kit is based on CPE antibody-CPE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CPE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CPE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CPE elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CPE in samples. An antibody specific for CPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPE bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for CPE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,256 Da
NCBI Official Full Name
carboxypeptidase E preproprotein
NCBI Official Synonym Full Names
carboxypeptidase E
NCBI Official Symbol
NCBI Official Synonym Symbols
CPH; fat; Cph1; Cph-1; R74677; NF-alpha1
  [Similar Products]
NCBI Protein Information
carboxypeptidase E
UniProt Protein Name
Carboxypeptidase E
UniProt Synonym Protein Names
Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CPE; CPH  [Similar Products]
NCBI Summary for CPE
This gene encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin. [provided by RefSeq, Jan 2016]
UniProt Comments for CPE
CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.10; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8 B3.1|8 32.3 cM

Cellular Component: cell soma; cytoplasmic vesicle; dendrite; extracellular region; extracellular space; Golgi apparatus; membrane; membrane raft; perikaryon; secretory granule; secretory granule membrane

Molecular Function: carboxypeptidase activity; cell adhesion molecule binding; cobalt ion binding; hydrolase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; neurexin family protein binding; peptidase activity; protein domain specific binding; serine carboxypeptidase activity; zinc ion binding

Biological Process: insulin processing; peptide catabolic process; peptide metabolic process; protein processing; proteolysis; Wnt signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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