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cPLA2 clia kit :: Human cPLA2 (Cytosolic Phospholipase A2) CLIA Kit

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Catalog # MBS2532062
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cPLA2 (Cytosolic Phospholipase A2), CLIA Kit

Popular Item
Full Product Name

Human cPLA2 (Cytosolic Phospholipase A2) CLIA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
600522
Species Reactivity
Specificity
This kit recognizes natural and recombinant cPLA2. No significant cross-reactivity or interference between cPLA2 and analogues was observed.
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of cPLA2 clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
cPLA2 clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of cPLA2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to cPLA2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for cPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain cPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of cPLA2. You can calculate the concentration of cPLA2 in the samples by comparing the RLU value of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of cPLA2 clia kit
cPLA2 clia kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2532062. Click to request current manual
NCBI/Uniprot data below describe general gene information for cPLA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
85,239 Da
NCBI Official Full Name
cytosolic phospholipase A2 isoform 2
NCBI Official Synonym Full Names
phospholipase A2, group IVA (cytosolic, calcium-dependent)
NCBI Official Symbol
PLA2G4A  [Similar Products]
NCBI Official Synonym Symbols
cPLA2; PLA2G4; cPLA2-alpha
  [Similar Products]
NCBI Protein Information
cytosolic phospholipase A2
UniProt Protein Name
Cytosolic phospholipase A2
UniProt Synonym Protein Names
Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase
UniProt Gene Name
PLA2G4A  [Similar Products]
UniProt Synonym Gene Names
CPLA2; PLA2G4; cPLA2  [Similar Products]
UniProt Entry Name
PA24A_HUMAN
NCBI Summary for cPLA2
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
UniProt Comments for cPLA2
cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: EC 3.1.1.5; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; EC 3.1.1.4; Phospholipase; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; Golgi apparatus; mitochondrial inner membrane; nucleus; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; histone acetyltransferase binding; lysophospholipase activity; phospholipase A2 activity

Biological Process: aging; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; decidualization; glycerophospholipid biosynthetic process; icosanoid biosynthetic process; icosanoid metabolic process; luteolysis; ovulation from ovarian follicle; phosphatidic acid biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; platelet activating factor biosynthetic process; platelet activation; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell proliferation; positive regulation of fever; positive regulation of prostaglandin biosynthetic process; positive regulation of vesicle fusion; response to calcium ion; response to glucocorticoid stimulus; response to heat; response to hydrogen peroxide; response to lipopolysaccharide; response to lithium ion; response to methylmercury; response to organic nitrogen; response to vitamin D; surfactant homeostasis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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