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cPLA2 recombinant protein :: Phospholipase A2, Cytosolic (cPLA2) Recombinant Protein

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Catalog # MBS2011220 (3 units are in stock [0.1 mg] for immediate shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $145 +1 FREE 8GB USB
  0.05 mg  /  $280 +1 FREE 8GB USB
  0.1 mg  /  $430 +1 FREE 8GB USB
  0.2 mg  /  $530 +1 FREE 8GB USB
  0.5 mg  /  $1,025 +1 FREE 8GB USB
  1 mg  /  $1,525 +1 FREE 8GB USB
Product Name

Phospholipase A2, Cytosolic (cPLA2), Recombinant Protein

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Full Product Name

Recombinant Phospholipase A2, Cytosolic (cPLA2)

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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2011220 Technical Datasheet
Chromosome Location
Chromosome: 1; NC_000001.10 (186798032..186958113). Location: 1q25
OMIM
600522
3D Structure
ModBase 3D Structure for P47712
Host
E Coli
Purity/Purification
>90%
Concentration
Original Concentration: 200ug/mL (lot specific)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Met1~Ser178
Tags
N-terminal His Tag
Subcellular Location
Cytoplasm, Chromosome
Buffer
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Predicted isoelectric point
5.9
Predicted Molecular Mass
21.9kDa
Accurate Molecular Mass
26kDa as determined by SDS-PAGE reducing conditions.
Traits
Freeze-dried powder.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of cPLA2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for cPLA2 recombinant protein
Positive Control. Immunogen, SDS-PAGE, Western Blot (WB).

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of cPLA2 recombinant protein
cPLA2 recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for cPLA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
cytosolic phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IVA (cytosolic, calcium-dependent)
NCBI Official Symbol
PLA2G4A  [Similar Products]
NCBI Official Synonym Symbols
PLA2G4; cPLA2-alpha
  [Similar Products]
NCBI Protein Information
cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein
UniProt Protein Name
Cytosolic phospholipase A2
UniProt Synonym Protein Names
Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase
UniProt Gene Name
PLA2G4A  [Similar Products]
UniProt Synonym Gene Names
CPLA2; PLA2G4; cPLA2  [Similar Products]
UniProt Entry Name
PA24A_HUMAN
NCBI Summary for cPLA2
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
UniProt Comments for cPLA2
cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: EC 3.1.1.5; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid; Phospholipase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activation; platelet activating factor biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation
Research Articles on cPLA2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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