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CPS1 elisa kit :: Mouse carbamoyl-phosphate synthetase 1, mitochondrial ELISA Kit

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Catalog # MBS920676
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Typical Testing Data/Standard Curve (for reference only)
Product Name

carbamoyl-phosphate synthetase 1, mitochondrial (CPS1), ELISA Kit

Popular Item
Full Product Name

Mouse Carbamoyl-phosphate synthase [ammonia], mitochondrial, CPS1 ELISA Kit

Product Synonym Names
Mouse Carbamoyl-phosphate synthase [ammonia]; mitochondrial (CPS1) ELISA kit; CPSase I; carbamoyl-phosphate synthetase 1; carbamoylphosphate synthetase I; carbamoyl-phosphate synthetase 1; mitochondrial
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS920676 Testing Data
3D Structure
ModBase 3D Structure for Q8C196
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CPS1. No significant cross-reactivity or interference between Mouse CPS1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
6.25 pg/ml-400 pg/ml
Sensitivity
1.56 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 3
Urea cycle
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CPS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CPS1purchase
MBS920676 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbamoyl-phosphate synthetase 1, mitochondrial (CPS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CPS1. The ELISA analytical biochemical technique of the MBS920676 kit is based on CPS1 antibody-CPS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CPS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CPS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CPS1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CPS1 elisa kit
CPS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS920676. Click to request current manual
NCBI/Uniprot data below describe general gene information for CPS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
164,618 Da
NCBI Official Full Name
carbamoyl-phosphate synthase
NCBI Official Synonym Full Names
carbamoyl-phosphate synthetase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
CPS; D1Ucla3; 4732433M03Rik
  [Similar Products]
NCBI Protein Information
carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthase [ammonia], mitochondrial; CPSase I; carbamoylphosphate synthetase I; carbamoyl-phosphate synthetase I
UniProt Protein Name
Carbamoyl-phosphate synthase [ammonia], mitochondrial
UniProt Synonym Protein Names
Carbamoyl-phosphate synthetase I; CPSase I
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CPSase I  [Similar Products]
UniProt Entry Name
CPSM_MOUSE
NCBI Summary for CPS1
This gene encodes a protein localized to the inner mitochondrial matrix. The encoded protein plays a role in the detoxification of ammonia by catalyzing the first step in the urea cycle in which carbomyl-phosphate is synthesized from ammonia and bicarbonate. Carbamoyl-phosphate is subsequently converted to urea that is excreted by the kidneys. Deficiency of the encoded enzyme leads to an accumulation of ammonia in the blood. High levels of ammonia are toxic to the central nervous system and result in neurological disorders. [provided by RefSeq, Oct 2013]
UniProt Comments for CPS1
CPS1: Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D). CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation. Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 6.3.4.16; Ligase; Energy Metabolism - nitrogen; Amino Acid Metabolism - arginine and proline; Nucleolus

Cellular Component: protein complex; mitochondrion; cytoplasm; mitochondrial inner membrane; nucleus

Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; protein binding; glutamate binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; metal ion binding; endopeptidase activity; nucleotide binding; protein complex binding; phospholipid binding; calcium ion binding; catalytic activity; ATP binding; ligase activity

Biological Process: nitrogen compound metabolic process; glycogen catabolic process; arginine biosynthetic process; metabolic process; glutamine catabolic process; triacylglycerol catabolic process; response to lipopolysaccharide; homocysteine metabolic process; positive regulation of vasodilation; proteolysis; nitric oxide metabolic process; anion homeostasis; urea cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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