• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CRAF elisa kit :: Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase ELISA Kit

Scan QR to view Datasheet
Catalog # MBS077112
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
CRAF elisa kit
Product Name

C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF), ELISA Kit

Popular Item
Full Product Name

Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q99N57
Species Reactivity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Undiluted original Human body fluids, tissue homogenates, secretions or feces samples.
Assay Type
Sandwich (Quantitative)
Detection Range
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CRAF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCRAFpurchase
MBS077112 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRAF. The ELISA analytical biochemical technique of the MBS077112 kit is based on CRAF antibody-CRAF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRAF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRAF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CRAF elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CRAF (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS077112. Click to request current manual
NCBI/Uniprot data below describe general gene information for CRAF. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,917 Da
NCBI Official Full Name
cRAF, partial
NCBI Official Synonym Full Names
v-raf-leukemia viral oncogene 1
NCBI Official Symbol
NCBI Official Synonym Symbols
Craf1; Raf-1; c-Raf; v-Raf; AA990557; BB129353; 6430402F14Rik; D830050J10Rik
  [Similar Products]
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase; cRaf; Craf1 transforming; proto-oncogene c-RAF; sarcoma 3611 oncogene; murine sarcoma 3611 oncogene 1; v-raf-1 leukemia viral oncogene 1
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Synonym Protein Names
Proto-oncogene c-RAF; cRaf; Raf-1
UniProt Gene Name
UniProt Synonym Gene Names
Craf; cRaf  [Similar Products]
UniProt Entry Name
UniProt Comments for CRAF
RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC; Kinase, protein; TKL group; RAF family

Cellular Component: Golgi apparatus; membrane; mitochondrion; cytoplasm; plasma membrane; pseudopodium; nucleus; cytosol

Molecular Function: identical protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; Ras GTPase binding; MAP kinase kinase kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; receptor signaling protein activity; kinase activity; ATP binding

Biological Process: activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; MAPKKK cascade; intermediate filament cytoskeleton organization and biogenesis; signal transduction; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of cell proliferation; response to hypoxia; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; negative regulation of protein complex assembly; phosphorylation; negative regulation of apoptosis
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

Contact Us

Please fill out the form below and our representative will get back to you shortly.