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CRH elisa kit :: Rat corticotropin releasing hormone ELISA Kit

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Catalog # MBS703710
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $590 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

corticotropin releasing hormone (CRH), ELISA Kit

Popular Item
Full Product Name

Rat corticotropin releasing hormone, CRH ELISA Kit

Product Synonym Names
Rat corticotropin releasing hormone; CRH ELISA Kit; CRF; corticotropin-releasing factor; corticotropin releasing hormone
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P01143
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat CRH. No significant cross-reactivity or interference between rat CRH and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Competitive
Detection Range
1.25 ng/ml -20 ng/ml
Sensitivity
Typically less than 1.25 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CRHpurchase
MBS703710 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the corticotropin releasing hormone (CRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRH. The ELISA analytical biochemical technique of the MBS703710 kit is based on CRH antibody-CRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CRH elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRH. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated CRH. A competitive inhibition reaction is launched between CRH (Standards or samples) and Biotin-conjugated CRH with the pre-coated antibody specific for CRH. The more amount of CRH in samples, the less antibody bound by Biotin-conjugated CRH. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of CRH in the sample. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CRH elisa kit
CRH elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for CRH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
20,680 Da
NCBI Official Full Name
corticoliberin
NCBI Official Synonym Full Names
corticotropin releasing hormone
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
corticoliberin; corticotropin-releasing factor; corticotropin-releasing hormone
UniProt Protein Name
Corticoliberin
UniProt Synonym Protein Names
Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CRF_RAT
NCBI Summary for CRH
binds CRH receptors and mediates neuroendocrine stress response; may play a role in the consolidation of long term memory [RGD, Feb 2006]
UniProt Comments for CRH
Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.1 PublicationManual assertion based on experiment in:Ref.3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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