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CRHBP elisa kit :: Human corticotropin releasing hormone binding protein ELISA Kit

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Catalog # MBS920581
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

corticotropin releasing hormone binding protein (CRHBP), ELISA Kit

Popular Item
Full Product Name

Human Corticotropin-releasing factor-binding protein, CRHBP ELISA Kit

Product Synonym Names
Human Corticotropin-releasing factor-binding protein (CRHBP) ELISA kit; CRF-BP; CRFBP; corticotropin releasing hormone-binding protein; corticotropin releasing hormone binding protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS920581 Testing Data
OMIM
122559
3D Structure
ModBase 3D Structure for P24387
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CRHBP. No significant cross-reactivity or interference between human CRHBP and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of human CRHBP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CRHBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCRHBPpurchase
MBS920581 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the corticotropin releasing hormone binding protein (CRHBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRHBP. The ELISA analytical biochemical technique of the MBS920581 kit is based on CRHBP antibody-CRHBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRHBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRHBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CRHBP elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRHBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRHBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRHBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRHBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CRHBP elisa kit
CRHBP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS920581. Click to request current manual
NCBI/Uniprot data below describe general gene information for CRHBP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,144 Da
NCBI Official Full Name
corticotropin-releasing factor-binding protein
NCBI Official Synonym Full Names
corticotropin releasing hormone binding protein
NCBI Official Symbol
CRHBP  [Similar Products]
NCBI Official Synonym Symbols
CRFBP; CRF-BP
  [Similar Products]
NCBI Protein Information
corticotropin-releasing factor-binding protein; CRH-BP; CRF-binding protein; corticotropin releasing hormone-binding protein; corticotropin-releasing hormone-binding protein
UniProt Protein Name
Corticotropin-releasing factor-binding protein
UniProt Synonym Protein Names
Corticotropin-releasing hormone-binding protein; CRH-BP
UniProt Gene Name
CRHBP  [Similar Products]
UniProt Synonym Gene Names
CRFBP; CRF-BP; CRF-binding protein; CRH-BP  [Similar Products]
UniProt Entry Name
CRHBP_HUMAN
NCBI Summary for CRHBP
Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]
UniProt Comments for CRHBP
CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: multivesicular body; Golgi apparatus; extracellular space; microtubule; endoplasmic reticulum; dendrite; perikaryon; intracellular; nerve terminal; nucleus; secretory granule

Molecular Function: protein binding; corticotropin-releasing hormone binding; peptide binding

Biological Process: negative regulation of adrenocorticotropic hormone secretion; synaptic transmission, dopaminergic; regulated secretory pathway; learning and/or memory; hormone-mediated signaling; cellular response to stress; regulation of adrenocorticotropin hormone secretion; hormone metabolic process; female pregnancy; behavioral response to ethanol; signal transduction; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with CRHBP elisa kit
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