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CRKL elisa kit :: Human v-crk sarcoma virus CT10 oncogene homolog (avian) -like ELISA Kit

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Catalog # MBS901280
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Typical Testing Data/Standard Curve (for reference only)
Product Name

v-crk sarcoma virus CT10 oncogene homolog (avian) -like (CRKL), ELISA Kit

Popular Item
Also Known As

Human Crk-like protein, CRKL ELISA Kit

Product Synonym Names
Human Crk-like protein (CRKL) ELISA kit; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian) -like
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS901280 Testing Data
Sequence Length
303
OMIM
602007
3D Structure
ModBase 3D Structure for P46109
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CRKL. No significant cross-reactivity or interference between Human CRKL and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CRKL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCRKLpurchase
MBS901280 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the v-crk sarcoma virus CT10 oncogene homolog (avian) -like (CRKL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRKL. The ELISA analytical biochemical technique of the MBS901280 kit is based on CRKL antibody-CRKL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRKL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRKL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CRKL elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRKL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRKL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CRKL elisa kit
CRKL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS901280. Click to request current manual
NCBI/Uniprot data below describe general gene information for CRKL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,777 Da
NCBI Official Full Name
crk-like protein
NCBI Official Synonym Full Names
v-crk avian sarcoma virus CT10 oncogene homolog-like
NCBI Official Symbol
NCBI Protein Information
crk-like protein
UniProt Protein Name
Crk-like protein
Protein Family
UniProt Gene Name
UniProt Entry Name
CRKL_HUMAN
NCBI Summary for CRKL
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
UniProt Comments for CRKL
CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: signal transducer activity; protein binding; SH3/SH2 adaptor activity

Biological Process: anterior/posterior pattern formation; blood vessel development; organ morphogenesis; nerve growth factor receptor signaling pathway; thymus development; activation of MAPKK activity; heart development; Ras protein signal transduction; JNK cascade; parathyroid gland development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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