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CSDA elisa kit :: Human cold shock domain protein A ELISA Kit

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Catalog # MBS946489
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cold shock domain protein A (CSDA), ELISA Kit

Full Product Name

Human DNA-binding protein A, CSDA ELISA Kit

Product Synonym Names
Human DNA-binding protein A (CSDA) ELISA kit; CSDA1; DBPA; ZONAB; DNA-binding protein A; ZO-1-associated nucleic acid-binding protein; cold-shock domain containing A1; cold-shock domain protein A; single-strand DNA-binding protein NF-GMB; cold shock domain protein A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS946489 Testing Data
OMIM
603437
3D Structure
ModBase 3D Structure for P16989
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CSDA. No significant cross-reactivity or interference between human CSDA and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
46.88 pg/ml -3000 pg/ml.
Sensitivity
The minimum detectable dose of human CSDA is typically less than 11.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CSDA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCSDApurchase
MBS946489 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cold shock domain protein A (CSDA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSDA. The ELISA analytical biochemical technique of the MBS946489 kit is based on CSDA antibody-CSDA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSDA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSDA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CSDA elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CSDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CSDA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSDA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSDA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CSDA elisa kit
CSDA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS946489. Click to request current manual
NCBI/Uniprot data below describe general gene information for CSDA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,090 Da
NCBI Official Full Name
Y-box-binding protein 3 isoform b
NCBI Official Synonym Full Names
Y box binding protein 3
NCBI Official Symbol
NCBI Official Synonym Symbols
CSDA; DBPA; CSDA1; ZONAB
  [Similar Products]
NCBI Protein Information
Y-box-binding protein 3; DNA-binding protein A; cold shock domain protein A; cold-shock domain protein A; cold-shock domain containing A1; cold shock domain-containing protein A; single-strand DNA-binding protein NF-GMB; ZO-1-associated nucleic acid-binding protein
UniProt Protein Name
Y-box-binding protein 3
UniProt Synonym Protein Names
Cold shock domain-containing protein A; DNA-binding protein A; Single-strand DNA-binding protein NF-GMB
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CSDA; DBPA  [Similar Products]
UniProt Entry Name
YBOX3_HUMAN
UniProt Comments for CSDA
NF-GMB: a member of the cold-shock domain (CSD) family. Two different splice-variant isoforms have been described.

Protein type: Transcription factor; RNA-binding

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: polysome; tight junction; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: Rho GTPase binding; mRNA 3'-UTR binding; double-stranded DNA binding; transcription factor activity; transcription corepressor activity; single-stranded DNA binding

Biological Process: negative regulation of skeletal muscle development; fertilization; transcription, DNA-dependent; in utero embryonic development; male gonad development; spermatogenesis; response to cold; negative regulation of transcription from RNA polymerase II promoter; positive regulation of organ growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withCSDA elisa kit
 Reference Product  PubMed Publications
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Pathways associated with CSDA elisa kit
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