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CSF2 elisa kit :: Mouse colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit

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Catalog # MBS700847
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $375 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,295 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,330 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

colony stimulating factor 2 (granulocyte-macrophage) (CSF2), ELISA Kit

Popular Item
Full Product Name

Mouse Granulocyte-Macrophage Colony Stimulating Factor, GM-CSF ELISA Kit

Product Synonym Names
Mouse Granulocyte-Macrophage Colony Stimulating Factor; GM-CSF ELISA Kit; GMCSF; MGC131935; MGC138897; colony stimulating factor 2; granulocyte-macrophage colony stimulating factor; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS700847 Testing Data
3D Structure
ModBase 3D Structure for P01587
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CSF2. No significant cross-reactivity or interference between Mouse CSF2 and analogues was observed.
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CSF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CSF2purchase
MBS700847 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the colony stimulating factor 2 (granulocyte-macrophage) (CSF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSF2. The ELISA analytical biochemical technique of the MBS700847 kit is based on CSF2 antibody-CSF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CSF2 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GM-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GM-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CSF2 elisa kit
CSF2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700847. Click to request current manual
NCBI/Uniprot data below describe general gene information for CSF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
16,091 Da
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
NCBI Official Synonym Symbols
Csfgm; GMCSF; Gm-CSf; MGI-IGM
  [Similar Products]
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF; put. GM-CSF; granulocyte-macrophage colony stimulating factor 2
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF
UniProt Gene Name
UniProt Synonym Gene Names
Csfgm; GM-CSF; CSF  [Similar Products]
UniProt Entry Name
CSF2_MOUSE
UniProt Comments for CSF2
Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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