• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CSF2 protein :: Granulocyte Macrophage Colony Stimulating Factor Protein

Scan QR to view Datasheet
Catalog # MBS546106
Unit / Price
Scan QR to view Datasheet
  0.005 mg  /  $165 +1 FREE 8GB USB
  0.02 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $2,150 +2 FREE 8GB USB
CSF2 protein
Product Name

Granulocyte Macrophage Colony Stimulating Factor (CSF2), Protein

Full Product Name

Recombinant Human GranulocyteMacrophage Colony Stimulating Factor (rHu GM-CSF)

Product Synonym Names
Granulocyte Macrophage Colony Stimulating Factor; human GM-CSF; Human Granulocyte-Macrophage Colony Stimulating Factor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 5; NC_000005.9 (131409485..131411863). Location: 5q31.1
OMIM
138960
3D Structure
ModBase 3D Structure for P04141
Host
E Coli
Purity/Purification
>96% by SDS-PAGE and HPLC analyses.
Form/Format
Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Molecular Weight Information
Approximately 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.
Endotoxin Level
Less than 1EU/mg of rHuGM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
Preparation and Storage
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of CSF2 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CSF2 protein
GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GMCSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells.
NCBI/Uniprot data below describe general gene information for CSF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,295 Da[Similar Products]
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
NCBI Official Synonym Symbols
GMCSF
  [Similar Products]
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF; Molgramostin; Sargramostim
UniProt Gene Name
UniProt Synonym Gene Names
GMCSF; GM-CSF; CSF  [Similar Products]
UniProt Entry Name
CSF2_HUMAN
NCBI Summary for CSF2
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
UniProt Comments for CSF2
GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; immune response; myeloid dendritic cell differentiation; positive regulation of DNA replication
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000