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CSF3 elisa kit :: Human G-CSF ELISA Kit

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Catalog # MBS175868
Unit / Price
  96-Strip-Wells  /  $305 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

G-CSF (CSF3), ELISA Kit

Popular Item
Also Known As

Human G-CSF PicoKine ELISA Kit

Product Synonym Names
Granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte); C17orf33; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; MGI IG; OTTMUSP00000006785; Pluripoietin; RP23-395E10.4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
207
OMIM
138970
3D Structure
ModBase 3D Structure for P09919
Species Reactivity
Specificity
Natural and recombinant human G-CSF
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 4pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CSF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCSF3purchase
MBS175868 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G-CSF (CSF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSF3. The ELISA analytical biochemical technique of the MBS175868 kit is based on CSF3 antibody-CSF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CSF3 elisa kit
Principle of the assay: human G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for G-CSF has been precoated onto 96-well plates. Standards(E.coli,T31-P204) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human G-CSF amount of sample captured in plate.
Background: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture.1 G-CSF is also termed colonystimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity.2 Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Statpathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant humanG-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

Typical Testing Data/Standard Curve (for reference only) of CSF3 elisa kit
Human G-CSF PicoKine ELISA Kit standard curve
CSF3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175868. Click to request current manual
NCBI/Uniprot data below describe general gene information for CSF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,434 Da
NCBI Official Full Name
Granulocyte colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 3 (granulocyte)
NCBI Official Symbol
NCBI Official Synonym Symbols
GCSF; CSF3OS; C17orf33
  [Similar Products]
NCBI Protein Information
granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Synonym Protein Names
Pluripoietin; INN: Filgrastim; INN: Lenograstim
UniProt Gene Name
UniProt Synonym Gene Names
C17orf33; GCSF; G-CSF  [Similar Products]
UniProt Entry Name
CSF3_HUMAN
NCBI Summary for CSF3
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
UniProt Comments for CSF3
G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of protein binding; positive regulation of myeloid cell differentiation; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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