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CSPG4 elisa kit :: Mouse Chondroitin sulfate proteoglycan 4 (CSPG4) ELISA Kit

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Catalog # MBS7229697
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Chondroitin sulfate proteoglycan 4 (CSPG4), ELISA Kit

Popular Item
Full Product Name

Mouse Chondroitin sulfate proteoglycan 4 (CSPG4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601172
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CSPG4. No significant cross-reactivity or interference between CSPG4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSPG4 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CSPG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCSPG4purchase
MBS7229697 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chondroitin sulfate proteoglycan 4 (CSPG4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSPG4. The ELISA analytical biochemical technique of the MBS7229697 kit is based on CSPG4 antibody-CSPG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSPG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSPG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CSPG4 elisa kit
Intended Uses: This CSPG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CSPG4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CSPG4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSPG4 antibody and an CSPG4-HRP conjugate. The assay sample and buffer are incubated together with CSPG4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSPG4 concentration since CSPG4 from samples and CSPG4-HRP conjugate compete for the anti-CSPG4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSPG4 from the sample, fewer sites are left to bind CSPG4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSPG4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CSPG4 elisa kit

Typical Standard Curve/Testing Data of CSPG4 elisa kit
CSPG4 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7229697. Click to request current manual
NCBI/Uniprot data below describe general gene information for CSPG4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
250,537 Da
NCBI Official Full Name
Chondroitin sulfate proteoglycan 4
NCBI Official Synonym Full Names
chondroitin sulfate proteoglycan 4
NCBI Official Symbol
CSPG4  [Similar Products]
NCBI Official Synonym Symbols
NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
  [Similar Products]
NCBI Protein Information
chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4 (melanoma-associated)
UniProt Protein Name
Chondroitin sulfate proteoglycan 4
UniProt Synonym Protein Names
Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan
UniProt Gene Name
CSPG4  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CSPG4_HUMAN
NCBI Summary for CSPG4
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]
UniProt Comments for CSPG4
NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: lysosomal lumen; focal adhesion; cell surface; integral to plasma membrane; Golgi lumen; apical plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein kinase binding

Biological Process: chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; tissue remodeling; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; carbohydrate metabolic process; chondroitin sulfate catabolic process; angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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