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CT elisa kit :: Porcine Calcitonin (CT) ELISA Kit

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Catalog # MBS262174
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Calcitonin (CT), ELISA Kit

Popular Item
Full Product Name

Porcine Calcitonin (CT) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
114130
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
1000 pg/ml-15.6 pg/ml
Sensitivity
Up to 5 pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CTpurchase
MBS262174 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcitonin (CT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CT. The ELISA analytical biochemical technique of the MBS262174 kit is based on CT antibody-CT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CT elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine CT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Standard Curve/Testing Data of CT elisa kit
CT elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS262174. Click to request current manual
NCBI/Uniprot data below describe general gene information for CT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13,898 Da
NCBI Official Full Name
calcitonin isoform CT preproprotein
NCBI Official Synonym Full Names
calcitonin-related polypeptide alpha
NCBI Official Symbol
CALCA  [Similar Products]
NCBI Official Synonym Symbols
CT; KC; CGRP; CALC1; CGRP1; CGRP-I
  [Similar Products]
NCBI Protein Information
calcitonin; calcitonin gene-related peptide 1; katacalcin; calcitonin 1; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha
UniProt Protein Name
Calcitonin
UniProt Synonym Protein Names
Calcitonin carboxyl-terminal peptide; CCP; PDN-21
UniProt Gene Name
CALCA  [Similar Products]
UniProt Synonym Gene Names
CALC1; CCP  [Similar Products]
UniProt Entry Name
CALC_HUMAN
NCBI Summary for CT
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]
UniProt Comments for CT
CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus

Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; protein amino acid phosphorylation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of interleukin-8 production; monocyte chemotaxis; leukocyte adhesion; elevation of cytosolic calcium ion concentration; cell-cell signaling; vasculature development; neuropeptide signaling pathway; regulation of blood pressure; negative regulation of smooth muscle contraction; receptor internalization; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; negative regulation of bone resorption; inflammatory response; positive regulation of macrophage differentiation; detection of temperature stimulus involved in sensory perception of pain; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with CT elisa kit
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