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Ctgf elisa kit :: Mouse Connective tissue growth factor ELISA Kit

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Catalog # MBS763358
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Connective tissue growth factor (Ctgf), ELISA Kit

Popular Item
Full Product Name

Mouse Connective tissue growth factor ELISA Kit

Product Synonym Names
Ctgf/IGFBP8/CCN2/HCS24/IGFBP-8/IBP-8/NOV2/Hypertrophic chondrocyte-specific protein 24/IGF-binding protein 8/CCN family member 2/Insulin-like growth factor-binding protein 8
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
121009
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Ctgf. No significant cross-reactivity or interference between Ctgf and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.6-1000pg/ml
Sensitivity
<9.375pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ctgf were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ctgf were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Ctgf elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Ctgfpurchase
MBS763358 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Connective tissue growth factor (Ctgf) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ctgf. The ELISA analytical biochemical technique of the MBS763358 kit is based on Ctgf antibody-Ctgf antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ctgf antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ctgf. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Ctgf elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Ctgf antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Ctgf antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Ctgf amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Ctgf can be calculated.

Typical Testing Data/Standard Curve (for reference only) of Ctgf elisa kit
Ctgf elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS763358. Click to request current manual
NCBI/Uniprot data below describe general gene information for Ctgf. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,205 Da
NCBI Official Full Name
connective tissue growth factor
NCBI Official Synonym Full Names
connective tissue growth factor
NCBI Official Symbol
NCBI Official Synonym Symbols
CCN2; NOV2; HCS24; IGFBP8
  [Similar Products]
NCBI Protein Information
connective tissue growth factor
UniProt Protein Name
Connective tissue growth factor
UniProt Synonym Protein Names
CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8
UniProt Gene Name
UniProt Synonym Gene Names
CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8  [Similar Products]
UniProt Entry Name
CTGF_HUMAN
NCBI Summary for Ctgf
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]
UniProt Comments for Ctgf
CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q23.1

Cellular Component: cell cortex; cis-Golgi network; cytosol; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; proteinaceous extracellular matrix

Molecular Function: fibronectin binding; growth factor activity; heparin binding; insulin-like growth factor binding; integrin binding; protein binding; protein C-terminus binding

Biological Process: angiogenesis; cartilage condensation; cell adhesion; cell differentiation; cell migration; cell-cell signaling; cell-matrix adhesion; cellular lipid metabolic process; epidermis development; fibroblast growth factor receptor signaling pathway; gene expression; integrin-mediated signaling pathway; lung development; organ senescence; ossification; positive regulation of caspase activity; positive regulation of cell activation; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of stress fiber formation; regulation of cell growth; regulation of chondrocyte differentiation; response to amino acid stimulus; response to anoxia; response to estradiol stimulus; response to glucose stimulus; response to mineralocorticoid stimulus; response to peptide hormone stimulus; response to wounding; signal transduction; tissue homeostasis; transcription initiation from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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