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CTNNa1 elisa kit :: Mouse CTNNa1 ELISA Kit

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Catalog # MBS2513712
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CTNNa1, ELISA Kit

Popular Item
Also Known As

Mouse CTNNa1 (Catenin, Alpha 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
906
OMIM
116805
3D Structure
ModBase 3D Structure for P35221
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse CTNNa1. No significant cross-reactivity or interference between Mouse CTNNa1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CTNNa1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCTNNa1purchase
MBS2513712 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CTNNa1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CTNNa1. The ELISA analytical biochemical technique of the MBS2513712 kit is based on CTNNa1 antibody-CTNNa1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CTNNa1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CTNNa1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CTNNa1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CTNNa1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTNNa1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CTNNa1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTNNa1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CTNNa1. You can calculate the concentration of CTNNa1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CTNNa1 elisa kit
CTNNa1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2513712. Click to request current manual
NCBI/Uniprot data below describe general gene information for CTNNa1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,550 Da
NCBI Official Full Name
catenin alpha-1 isoform 1
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), alpha 1, 102kDa
NCBI Official Symbol
CTNNA1  [Similar Products]
NCBI Official Synonym Symbols
CAP102
  [Similar Products]
NCBI Protein Information
catenin alpha-1; alpha E-catenin; alpha-E-catenin; alpha-catenin; alphaE-catenin; cadherin-associated protein,102kDa; renal carcinoma antigen NY-REN-13
UniProt Protein Name
Catenin alpha-1
UniProt Synonym Protein Names
Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13
Protein Family
UniProt Gene Name
CTNNA1  [Similar Products]
UniProt Entry Name
CTNA1_HUMAN
UniProt Comments for CTNNa1
CTNNA1: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F- actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Expressed ubiquitously in normal tissues. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: focal adhesion; lamellipodium; acrosome; plasma membrane; zonula adherens; catenin complex; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; cadherin binding; gamma-catenin binding; beta-catenin binding; structural molecule activity; vinculin binding

Biological Process: intercellular junction assembly and maintenance; apical junction assembly; gap junction assembly; protein heterooligomerization; axon regeneration; male gonad development; establishment and/or maintenance of cell polarity; actin filament organization; odontogenesis of dentine-containing teeth; muscle cell differentiation; negative regulation of neuroblast proliferation; ovarian follicle development; response to estrogen stimulus; positive regulation of smoothened signaling pathway; positive regulation of muscle cell differentiation; vascular endothelial growth factor receptor signaling pathway; cell adhesion; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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