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CTRC elisa kit :: Monkey chymotrypsin C (caldecrin) ELISA Kit

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Catalog # MBS706463
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Typical Testing Data/Standard Curve (for reference only)
Product Name

chymotrypsin C (caldecrin) (CTRC), ELISA Kit

Popular Item
Also Known As

Monkey Chymotrypsin-C, CTRC ELISA Kit

Product Synonym Names
Monkey Chymotrypsin-C (CTRC) ELISA kit; CLCR; ELA4; caldecrin; chymotrypsin C; elastase 4; elastase IV; serum calcium decreasing factor; chymotrypsin C (caldecrin)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS706463 Testing Data
Sequence Length
268
OMIM
601405
3D Structure
ModBase 3D Structure for Q99895
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Monkey CTRC. No significant cross-reactivity or interference between Monkey CTRC and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 ng/ml-2000 ng/ml
Sensitivity
7.81 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CTRC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCTRCpurchase
MBS706463 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chymotrypsin C (caldecrin) (CTRC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CTRC. The ELISA analytical biochemical technique of the MBS706463 kit is based on CTRC antibody-CTRC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CTRC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CTRC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CTRC elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTRC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTRC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTRC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTRC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CTRC elisa kit
CTRC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS706463. Click to request current manual
NCBI/Uniprot data below describe general gene information for CTRC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,484 Da
NCBI Official Full Name
chymotrypsin-C preproprotein
NCBI Official Synonym Full Names
chymotrypsin C (caldecrin)
NCBI Official Symbol
NCBI Official Synonym Symbols
CLCR; ELA4
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NCBI Protein Information
chymotrypsin-C; caldecrin; elastase 4; elastase IV; serum calcium decreasing factor
UniProt Protein Name
Chymotrypsin-C
UniProt Synonym Protein Names
Caldecrin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CTRC_HUMAN
NCBI Summary for CTRC
This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for CTRC
Function: Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.1 PublicationManual assertion based on experiment in:Ref.8

Catalytic activity: Preferential cleavage: Leu-|-Xaa, Tyr-|-Xaa, Phe-|-Xaa, Met-|-Xaa, Trp-|-Xaa, Gln-|-Xaa, Asn-|-Xaa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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