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Medium 199 w/ Earle's w/o L-Glutamine Culture Media

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Catalog # MBS653449
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  25 L  /  $570 +1 FREE 8GB USB
Medium 199 w/ Earle's w/o L-Glutamine culture media
Product Name

Medium 199 w/ Earle's w/o L-Glutamine, Culture Media

Popular Item
Full Product Name

Medium 199 w/ Earle's w/o L-Glutamine (Powder, TC 199)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Cell Culture Grade
Light pink, homogenous, free flowing powder
Endotoxin Level
<= 1EU/ml
Yellow, clear, complete
Directions per Liter
Dissolve 9.4g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 0.35g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-liable compounds that can be damaged with autoclaving.
Components shown as mg/liter:
Amino Acids
L-Alanine: 25
L-Arginine•HCl: 70
L-Aspartic Acid: 30
L-Cysteine•HCl•H2O: 0.11
L-Cystine•2HCl: 26
L-Glutamic Acid: 75
Glycine: 50
L-Histidine•HCl•H2O: 21.88
Hydroxy-L-Proline: 10
L-Isoleucine: 20
L-Leucine: 60
L-Lysine•HCl: 70
L-Methionine: 15
L-Phenylalanine: 25
L-Proline: 40
L-Serine: 25
L-Threonine: 30
L-Tryptophan: 10
L-Tyrosine•2Na•2H2O: 57.66
L-Valine 25
Inorganic Salts
Calcium Chloride: 200
Ferric Nitrate•9H2O: 0.72
Magnesium Sulfate: 97.7
Potassium Chloride: 400
Sodium Acetate: 50
Sodium Chloride: 6800
Sodium Phosphate Mono: 140
Adenine Sulfate: 10
p-Aminobenzoic Acid: 0.05
L-Ascorbic Acid: 0.05
D-Biotin: 0.01
Choline Chloride: 0.5
Calciferol: 0.1
Folic Acid: 0.01
i-Inositol: 0.05
Menadione, NaBisulfate•3H2O: 0.019
Niacinamide: 0.025
D-Pantothenic Acid•Ca: 0.01
Pyridoxine•HCl: 0.05
Riboflavin: 0.01
Thiamine•HCl: 0.01
DL-a-Tocopherol•PO4•2Na: 0.01
Vitamin A 9Retinol) Acetate: 0.14
5'-Adenylic acid: 0.2
Adenosine Triphosphate•2Na: 1
Cholesterol: 0.2
2'-Deoxy-D-ribose: 0.5
Thymine: 0.3
D-Glucose: 1000
Glutathione: 0.05
Guanine•HCl: 0.3
Hypoxanthine (Na): 0.35
Phenol Red, Sodium: 10
Tween 80: 5
D-Ribose: 0.5
Uracil: 0.3
Xanthine•Na: 0.34
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Other Notes
Small volumes of Medium 199 w/ Earle's w/o L-Glutamine culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
In 1950, Morgan, Morton and Parker described one of the first totally defined media that did not depend largely on animal products or extracts as nutritive sources. This medium supported the growth of primary chick embryo heart cells. It has since become known as Medium 199. Its current use is with the addition of serum in virus and vaccine production, and in the culture of many non-transformed cells. It is also used in combination with less complex media. Medium 199 modified with Earle's Balanced Salts is designed for use with cells maintained in ambient (non-CO2) atmospheric conditions. M2852 differs from the original formulation by containing all L-amino acids. The original formulation contained 10 DL-amino acids, which are less biologically active on a per gram basis than the corresponding L-amino acids.

The search for a synthetic medium to replace serum for maintaining cells in vitro began in the late nineteenth century and continues to this day. Ringer, Locks and Tyrode substituted physiological salt solutions augmented with glucose for serum and thereby laid the foundation for the development of defined media. As biochemical and analytical techniques have improved, more of the components in serum such as vitamins, hormones, and amino acids have been identified and incorporated into physiological salt solutions, reducing, and in some cases eliminating the concentrations of animal sera required as a medium supplement. Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago. Earle's Balanced Salts selection is strongly influenced by 1. type of cell, 2. type of culture monolayer, suspension, clonal, and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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