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RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red Culture Media

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Catalog # MBS653421
Unit / Price
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  10 L  /  $195 +1 FREE 8GB USB
  25 L  /  $350 +1 FREE 8GB USB
  50 L  /  $570 +1 FREE 8GB USB
  100 L  /  $830 +1 FREE 8GB USB
RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red culture media
Product Name

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red, Culture Media

Popular Item
Full Product Name

RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red (Powder)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Specificity
RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et al. at Roswell Park Memorial Institute.
Purity/Purification
Cell Culture Grade
Appearance
White, homogeneous, free flowing powder.
Solubility
Colorless, clear, complete
pH
8.3
Endotoxin
< = 1 EU/ml
Directions per Liter
Dissolve 7.4g in 800-900mL of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medioum to 0.1-0.3 pH unit below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 um membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Inorganic Salts
Calcium Nitrate•4H2O 0.1
Magnesium Sulfate 0.04884
Potassium Chloride 0.4
Sodium Chloride 6
Sodium Phosphate Dibasic 0.8
Amino Acids
L-Arginine Absent
L-Aspartic Acid Absent
L-Asparagine Absent
L-Cystine•2HCl Absent
L-Glutamic Acid Absent
L-Glutamine Absent
Glycine Absent
L-Histidine Absent
Hydroxy-L-proline Absent
L-Isoleucine Absent
L-Leucine Absent
L-Lysine•HCl Absent
L-Methionine Absent
L-Phenylalanine Absent
L-Proline Absent
L-Serine Absent
L-Threonine Absent
L-Tryptophan Absent
L-Tyrosine•2Na•2H2O Absent
L-Valine Absent
Vitamins
P-Amino Benzoic Acid 0.001
D-Biotin 0.0002
Choline Chloride 0.003
Vitamin B-12 0.000005
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.00025
Pyridoxine•HCl 0.001
Riboflavin 0.0002
Thiamine•HCl 0.001
Other
Glucose Absent
Glutathione 0.001
Phenol Red, Sodium Absent
Total
7.4g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time
Other Notes
Small volumes of RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. 
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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