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RPMI 1640 Medium w/o L-Glutamine, Calcium Free Culture Media

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Catalog # MBS652645
Unit / Price
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  10 L  /  $195 +1 FREE 8GB USB
  25 L  /  $350 +1 FREE 8GB USB
  50 L  /  $570 +1 FREE 8GB USB
  100 L  /  $830 +1 FREE 8GB USB
RPMI 1640 Medium w/o L-Glutamine, Calcium Free culture media
Product Name

RPMI 1640 Medium w/o L-Glutamine, Calcium Free, Culture Media

Popular Item
Full Product Name

RPMI 1640 Medium w/o L-Glutamine, Calcium Free (Powder)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Endotoxin Level
<= 1EU/mL
Appearance
Orange, homogeneous, free flowing powder
pH
8.7
Solubility
Magenta, clear, complete
Directions per Liter
Dissolve 10.04g in 900ml of ddH20, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.

Note: It may be necessary to lower the pH to 4.0 with 1 N HCI to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Inorganic Salts
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.4
Sodium Chloride: 6
Sodium Phosphate Dibasic: 0.8
Powder
Components shown as g/Liter Amino Acids:
L-ArginineHCI: 0.242
L-Asparagine•H20: 0.0568
L-Aspartic Acid: 0.02
L-Cystine•2HCI: 0.0652
L-Glutamic Acid: 0.02
Glycine: 0.01
L-Histidine•HCI•H20: 0.020
Hydroxy-L -pro line: 0.02
L –Isoleucine: 0.05
L-Leucine: 0.05
L-Lysine•HCI: 0.04
L-Methionine: 0.015
L-Phenylalanine: 0.0l5
L-Proline: 0.02
L-Serine: 0.03
L-Threonine: 0.02
L-Tryptophan: 0.005
L-Tyrosine•HCI: 0.024
L-Valine: 0.02

Vitamins:
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid, Na: 0.00025
Pyridoxine•HCI: 0.001
Riboflavin: 0.0002
Thiamine•HCI: 0.001

Other:
D-Glucose: 2.0
Glutathione: 0.001
Phenol Red, Sodium: 0.005

Total:
10.04g/Liter
Preparation and Storage
Store powder media in Room Temperature. Opened bottles should be capped tighly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Other Notes
Small volumes of RPMI 1640 Medium w/o L-Glutamine, Calcium Free culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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