Custom goat antibody services and goat antibody production
Please browse the packages below as an example of what we can do for you and contact CustomAntibody@mybiosource.com for more information or a customized quote.
Custom antibody production orders can be submitted online here. If you wish to download an order form, please click here.
|Options & Features||#MBS42-1P2G
(1 peptide-2 goats)
(2 peptides-2 goats)
|Immunizing peptide design||Yes||Yes|
|Peptide conjugation and immunization||Yes||Yes|
|Number of antigens||1||2|
|Number of goats||2||2|
|Number of antigen purified antibodies||2||4|
|Antigen ELISA titre determination||Yes||Yes|
|Extended protocol for multiple harvests||Optional||Optional|
|Conjugation to reporter||Optional||Optional|
|WB testing (Inquire)||Optional||Optional|
|Delivery with immunizing peptide and report||Yes||Yes|
We are specialists in antigen affinity purified polyclonal antibodies and our processes have been optimized during the past decade. Below are some of our advantages:
- LARGE BATCHES (Using goat as host means large batch sizes - remember that the costs to validate a small batch of antibody or a large one are identical.)
- DESIGN ADVICE (From a team with over a decade of experience.)
- PROFESSIONALISM (ISO9001 certified production laboratory. Highly educated and trained staff with leadership at PhD level. Fully qualified veterinary doctor on staff.)
Custom Antibody Services - FAQ's:• What is the expected yield per goat?
Yield strongly depends on the target protein, the individual goat, the choice of peptide and environmental factors. Yield can be as low as a few mg and as high as a few hundreds of mg. We never exceeded 400 mg levels from one single goat, except when using extended protocol . Please bear in mind that these figures are based on peptide affinity purified IgG developed to a 10-15aa peptide, so such yields cannot be fairly compared with those developed to total proteins, or with protein A/G purified antibodies.• Will you be able to provide grams of affinity purified IgG?
See above. For grams we will need to immunize several animals and pool the yield. It would make sense to order #MBS42-1P2G or #MBS42-2P2G before ordering grams of the same. Please bear in mind that each animal may produce a slightly different product. By pooling the products from several animals (all immunized with the same peptide) we would dilute any individual variations and the blend will be the gold standard for future productions. See Extended protocol for multiple harvests.• Why do you only test ELISA titres and not specificity?
The ELISA titre is merely a measure to test the reactivity of the product to the used immunogen. It does not reflect its reactivity to the target protein in tissues or cell lines, nor does it measure specificity. These need to be addressed in the applications that the custom antibody is required for. Both titre and specificity will be different for each application. Our titres are specified on the product sheets as: ELISA: antibody detection limit dilution.