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CX3CL1 recombinant protein :: CX3CL1/Fractalkine Recombinant Protein

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Catalog # MBS203381
Unit / Price
  0.1 mg  /  $510 +1 FREE 8GB USB
  0.5 mg  /  $1,335 +1 FREE 8GB USB
SDS-Page
Product Name

CX3CL1/Fractalkine, Recombinant Protein

Also Known As

CX3CL1/Fractalkine, 25- 100aa, Human, His tag, E Coli

Product Synonym Names
Chemokine (C-X3-C motif) ligand 1; ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1; CX3CL1; Chemokine (C-X3-C motif) ligand 1; small inducible cytokine subfamily D (Cys-X3-Cys) member 1 (fractalkine; neurotactin); fractalkine; neurotactin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
397
Sequence
MGSSHHHHHH SSGLVPRGSH MQHHGVTKCN ITCSKMTSKI PVALLIHYQQ NQASCGKRAI ILETRQHRLF CADPKEQWVK DAMQHLDRQA AALTRNG
OMIM
U91835 mRNA
3D Structure
ModBase 3D Structure for P78423
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4), 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of CX3CL1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CX3CL1 recombinant protein
CX3CL1, also known as fractalkine. CX3CL1 has a unique CX3C cysteine motif near the amino terminus and is the first member of a fourth branch of the chemokine superfamily. unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin like stalk. It is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Also, CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Recombinant human CX3CL1, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for CX3CL1 recombinant protein
Applications Tested/Suitable for CX3CL1 recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for CX3CL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10.9 kDa (97aa), confirmed by MALDI-TOF. [Similar Products]
NCBI Official Full Name
fractalkine isoform 1
NCBI Official Synonym Full Names
C-X3-C motif chemokine ligand 1
NCBI Official Symbol
CX3CL1  [Similar Products]
NCBI Official Synonym Symbols
NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
  [Similar Products]
NCBI Protein Information
fractalkine
UniProt Protein Name
Fractalkine
UniProt Synonym Protein Names
C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
Protein Family
UniProt Gene Name
CX3CL1  [Similar Products]
UniProt Synonym Gene Names
FKN; NTT; SCYD1  [Similar Products]
NCBI Summary for CX3CL1
This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]
UniProt Comments for CX3CL1
Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 (PubMed:23125415, PubMed:9931005, PubMed:21829356). Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350).
Product References and Citations for CX3CL1 recombinant protein
Bazan JF., et al. (1997) Nature. 385 (6617):640-4. ; Papadopoulos EJ., et al. (1999) Eur J Immunol. 29 (8):2551-9. ;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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