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CX40 recombinant protein :: Connexin 40 (CX40) Recombinant Protein

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Catalog # MBS2010997 (3 units are in stock [0.1 mg] for immediate shipment)
Unit / Price
  0.01 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $250 +1 FREE 8GB USB
  0.1 mg  /  $385 +1 FREE 8GB USB
  0.2 mg  /  $470 +1 FREE 8GB USB
  0.5 mg  /  $910 +1 FREE 8GB USB
  1 mg  /  $1,345 +1 FREE 8GB USB
Product Name

Connexin 40 (CX40), Recombinant Protein

Popular Item
Also Known As

Recombinant Connexin 40 (CX40)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_005101.3 (218648368..218669354). Location: 2q34
3D Structure
ModBase 3D Structure for P28234
Host
E Coli
Species Reactivity
Rat
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Source
Prokaryotic expression
Original Concentration
200ug/mL
Traits
Freeze-dried powder
Residues
Ala50~Glu303
Tags
N-terminal His-Tag
Tissue Specificity
Lung
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction
Predicted isoelectric point
6.9
Predicted Molecular Mass
30.1kDa
Accurate Molecular Mass
20&16kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation,glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for two years.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of CX40 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for CX40 recombinant protein
SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
Application Notes for CX40 recombinant protein
May be suitable for use in other assays to be determined by the end user.

Sequence Information of CX40 recombinant protein
CX40 recombinant protein Sequence Information image
Testing Data of CX40 recombinant protein
CX40 recombinant protein Testing Data image
NCBI/Uniprot data below describe general gene information for CX40. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
gap junction alpha-5 protein
NCBI Official Synonym Full Names
gap junction protein, alpha 5
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
gap junction alpha-5 protein; connexin 40; connexin-40; gap junction membrane channel protein alpha 5
UniProt Protein Name
Gap junction alpha-5 protein
UniProt Synonym Protein Names
Connexin-40
UniProt Gene Name
UniProt Synonym Gene Names
Cxn-40; Cx40  [Similar Products]
UniProt Entry Name
CXA5_RAT
NCBI Summary for CX40
forms intercellular connoxin channels in gap junctions; mediates cell-cell communication [RGD, Feb 2006]
UniProt Comments for CX40
GJA5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA5 are a cause of familial atrial standstill (FAS). Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Defects in GJA5 are the cause of familial atrial fibrillation type 11 (ATFB11). ATFB11 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Motility/polarity/chemotaxis; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: connexon complex; cell projection; integral to plasma membrane; gap junction

Molecular Function: gap junction hemi-channel activity; gap junction channel activity

Biological Process: blood vessel development; negative regulation of glomerular filtration; gap junction assembly; regulation of vasodilation; heart development; positive regulation of vasodilation; protein oligomerization; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure; regulation of systemic arterial blood pressure by renal system process; artery morphogenesis; embryonic heart tube development; negative regulation of blood pressure; angiogenesis; transmembrane transport; skeletal development; potassium ion transport; embryonic limb morphogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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