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CXCL17 elisa kit :: Human chemokine (C X C motif) ligand 17 (CXCL17) ELISA Kit

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Catalog # MBS7239012
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
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Typical Standard Curve/Testing Data
Product Name

chemokine (C X C motif) ligand 17 (CXCL17), ELISA Kit

Popular Item
Full Product Name

Human chemokine (C X C motif) ligand 17 (CXCL17) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
601393
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CXCL-17. No significant cross-reactivity or interference between CXCL-17 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCL-17 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000 pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CXCL17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCXCL17purchase
MBS7239012 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chemokine (C X C motif) ligand 17 (CXCL17) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL17. The ELISA analytical biochemical technique of the MBS7239012 kit is based on CXCL17 antibody-CXCL17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CXCL17 elisa kit
Intended Uses: This CXCL-17 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CXCL-17. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CXCL-17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CXCL-17 antibody and an CXCL-17-HRP conjugate. The assay sample and buffer are incubated together with CXCL-17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL-17 concentration since CXCL-17 from samples and CXCL-17-HRP conjugate compete for the anti-CXCL-17 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL-17 from the sample, fewer sites are left to bind CXCL-17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL-17 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CXCL17 elisa kit

Typical Standard Curve/Testing Data of CXCL17 elisa kit
CXCL17 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7239012. Click to request current manual
NCBI/Uniprot data below describe general gene information for CXCL17. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,248 Da
NCBI Official Full Name
chemokine
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 15
NCBI Official Symbol
CCL15  [Similar Products]
NCBI Official Synonym Symbols
LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 15; leukotactin 1; chemokine CC-2; new CC chemokine 3; small-inducible cytokine A15; macrophage inflammatory protein 5; small inducible cytokine subfamily A (Cys-Cys), member 15
UniProt Protein Name
C-C motif chemokine 15
UniProt Synonym Protein Names
Chemokine CC-2; HCC-2; Leukotactin-1; LKN-1; MIP-1 delta; Macrophage inflammatory protein 5; MIP-5; Mrp-2b; NCC-3; Small-inducible cytokine A15Cleaved into the following 3 chains:CCL15(22-92); CCL15(25-92); CCL15(29-92)
UniProt Gene Name
CCL15  [Similar Products]
UniProt Synonym Gene Names
MIP5; NCC3; SCYA15; HCC-2; LKN-1; MIP-5  [Similar Products]
UniProt Entry Name
CCL15_HUMAN
NCBI Summary for CXCL17
This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]
UniProt Comments for CXCL17
CCL15: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; chemoattractant activity; receptor binding

Biological Process: cellular calcium ion homeostasis; cell-cell signaling; positive chemotaxis; immune response; signal transduction; chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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