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CXCL5 elisa kit :: Mouse CXCL5/ENA-78 ELISA Kit

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Catalog # MBS176511
Unit / Price
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  96-Strip-Wells  /  $390 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CXCL5/ENA-78, ELISA Kit

Popular Item
Full Product Name

Mouse CXCL5/ENA-78 PicoKine ELISA Kit

Product Synonym Names
C-X-C motif chemokine 5; chemokine (C-X-C motif) ligand 5; AMCF II; AMCFII; C X C motif chemokine 5; Chemokine (C X C motif) ligand 5; CXCL 5; ENA 78; ENA78; Epithelial derived neutrophil activating protein 78; GCP 2; LIX; Neutrophil activating peptide ENA 78; Neutrophil activating protein 78; SCYB5; Scyb6; Small inducible cytokine B5; Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78); Small inducible cytokine subfamily B, member 15
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P50228
Species Reactivity
Specificity
Natural and recombinant mouse CXCL5
Samples
Cell culture supernates, serum and plasma(heparin1 EDTA, citrate}.
Assay Type
Quantitative Sandwich
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CXCL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CXCL5purchase
MBS176511 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CXCL5/ENA-78, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL5. The ELISA analytical biochemical technique of the MBS176511 kit is based on CXCL5 antibody-CXCL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CXCL5 elisa kit
Principle of the Assay: The mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.

Background/Introduction: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-y. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Typical Testing Data/Standard Curve (for reference only) of CXCL5 elisa kit
Mouse CXCL5/ENA-78 PicoKine ELISA Kit standard curve
CXCL5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS176511. Click to request current manual
NCBI/Uniprot data below describe general gene information for CXCL5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,235 Da
NCBI Official Full Name
C-X-C motif chemokine 5
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 5
NCBI Official Symbol
Cxcl5  [Similar Products]
NCBI Official Synonym Symbols
LIX; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
  [Similar Products]
NCBI Protein Information
C-X-C motif chemokine 5; cytokine LIX; small-inducible cytokine B5; granulocyte chemotactic protein-2; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B, member 15
UniProt Protein Name
C-X-C motif chemokine 5
UniProt Synonym Protein Names
Cytokine LIX; Small-inducible cytokine B5Cleaved into the following 2 chains:GCP-2(1-78); GCP-2(9-78)
Protein Family
UniProt Gene Name
Cxcl5  [Similar Products]
UniProt Synonym Gene Names
Scyb5  [Similar Products]
UniProt Entry Name
CXCL5_MOUSE
NCBI Summary for CXCL5
This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils and to have homeostatic and inflammatory functions. In mouse, deficiency of this gene is associated with increased lung inflammation that is neutrophil-dependent. [provided by RefSeq, May 2013]
UniProt Comments for CXCL5
CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; organ regeneration; positive regulation of JAK-STAT cascade; cytokine production; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; inflammatory response; chemotaxis; regulation of chemokine production; leukocyte homeostasis; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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